Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover

Jianmin Gao, Haibo Weng, Daheng Zhu, Mingxue Yuan, Fangxia Guan, Yu Xi

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

The production of extracellular cellulases by a newly isolated thermoacidophilic fungus, Aspergillus terreus M11, on the lignocellulosic materials was studied in solid-state fermentation (SSF). The results showed that the high-level cellulase activity was produced at 45 °C pH 3 and moisture 80% with corn stover and 0.8% yeast extract as carbon and nitrogen sources. 581 U endoglucanase activity, 243 U filter paper activity and 128 U β-glucosidase activity per gram of carbon source were obtained in the optimal condition. Endoglucanase and β-glucosidase exhibited their maximum activity at pH 2 and pH 3, respectively, and both of them showed remarkable stability in the range of pH 2-5. The activities of endoglucanase and β-glucosidase were up to the maximum at 70 °C and maintained about 65% and 53% of their original activities after incubation at 70 °C for 6 h. The enzyme preparations from this strain were used to hydrolyze Avicel. Higher hydrolysis yields of Avicel were up to 63% on 5% Avicel (w/v) for 72 h with 20 U FPase/g substrate.

Original languageEnglish (US)
Pages (from-to)7623-7629
Number of pages7
JournalBioresource Technology
Volume99
Issue number16
DOIs
StatePublished - Nov 2008
Externally publishedYes

Fingerprint

Aspergillus terreus
Cellulase
corn stover
Aspergillus
Glucosidases
endo-1,4-beta-glucanase
glucosidases
Zea mays
Enzymes
maize
Cellulose
enzyme
carbon
enzymes
fermentation
yeast
hydrolysis
Carbon
incubation
moisture

Keywords

  • Aspergillus terreus M11
  • Corn stover
  • Solid-state fermentation (SSF)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. / Gao, Jianmin; Weng, Haibo; Zhu, Daheng; Yuan, Mingxue; Guan, Fangxia; Xi, Yu.

In: Bioresource Technology, Vol. 99, No. 16, 11.2008, p. 7623-7629.

Research output: Contribution to journalArticle

Gao, Jianmin ; Weng, Haibo ; Zhu, Daheng ; Yuan, Mingxue ; Guan, Fangxia ; Xi, Yu. / Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. In: Bioresource Technology. 2008 ; Vol. 99, No. 16. pp. 7623-7629.
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