Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry

Hua Wang, David G. Castner, Buddy D. Ratner, Shaoyi Jiang

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Static time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful surface analysis technique for the characterization of protein films because of its chemical selectivity and surface sensitivity. In this study, static ToF-SIMS and principal component analysis (PCA), a multivariate data analysis method, were combined to probe the orientation of surface-immobilized immunoglobulin G (IgG). IgG orientation can enhance its ability to detect its antigen in immunoassay techniques. The IgG used in this work is the mouse monoclonal anti-human chorionic gonadotropin (anti-hCG). Anti-hCG films on different well-defined substrates have been studied using its F(ab′)2 and Fc fragments as references. Atomic force microscopy was used to characterize these protein films before static ToF-SIMS analysis. The results from PCA of ToF-SIMS spectra were related to the antibody primary amino acid composition and its three-dimensional structure.

Original languageEnglish (US)
Pages (from-to)1877-1887
Number of pages11
JournalLangmuir
Volume20
Issue number5
DOIs
StatePublished - Mar 2 2004
Externally publishedYes

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Secondary ion mass spectrometry
secondary ion mass spectrometry
Immunoglobulin G
principal components analysis
Principal component analysis
proteins
Proteins
Immunoglobulin Fc Fragments
immunoassay
Surface analysis
antigens
Chorionic Gonadotropin
Antigens
antibodies
Antibodies
mice
amino acids
Amino acids
Atomic force microscopy
selectivity

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

Cite this

Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry. / Wang, Hua; Castner, David G.; Ratner, Buddy D.; Jiang, Shaoyi.

In: Langmuir, Vol. 20, No. 5, 02.03.2004, p. 1877-1887.

Research output: Contribution to journalArticle

Wang, Hua ; Castner, David G. ; Ratner, Buddy D. ; Jiang, Shaoyi. / Probing the orientation of surface-immobilized immunoglobulin G by time-of-flight secondary ion mass spectrometry. In: Langmuir. 2004 ; Vol. 20, No. 5. pp. 1877-1887.
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