Probing the force generation and stepping behavior of cytoplasmic dynein

Arne Gennerich; Samara L. Reck-Peterson

doi:10.1007/978-1-61779-282-3_4

Probing the force generation and stepping behavior of cytoplasmic dynein

Arne Gennerich, Samara L. Reck-Peterson

Anatomy & Structural Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

11 Scopus citations

Abstract

Cytoplasmic dynein, which is the largest and arguably the most complex cytoskeletal motor protein, plays fundamental roles during cell division, nuclear positioning, and organelle and mRNA transport, by generating force and movement toward the minus ends of microtubules. Consequently, dynein is central to many physiological processes, and its dysfunction is implicated in human diseases. However, the molecular mechanism by which dynein produces force and movement remains poorly understood. Here, we describe the use of optical tweezers to probe the nanometer-scale motion and force generation of individual dynein molecules, and provide a hands-on protocol for how to purify cytoplasmic dynein from budding yeast in amounts sufficient for single-molecule studies.

Original language	English (US)
Title of host publication	Single Molecule Analysis
Subtitle of host publication	Methods and Protocols
Editors	Erwin J.G. Peterman, Gijs J.L. Wuite
Pages	63-80
Number of pages	18
DOIs	https://doi.org/10.1007/978-1-61779-282-3_4
State	Published - Oct 24 2011

Publication series

Name	Methods in Molecular Biology
Volume	783
ISSN (Print)	1064-3745

Keywords

Budding yeast
Cytoplasmic dynein
Microtubules
Molecular motors
Optical trapping
Optical tweezers
Saccharomyces cerevisiae
Single-molecule assays

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-61779-282-3_4

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Gennerich, A., & Reck-Peterson, S. L. (2011). Probing the force generation and stepping behavior of cytoplasmic dynein. In E. J. G. Peterman, & G. J. L. Wuite (Eds.), Single Molecule Analysis: Methods and Protocols (pp. 63-80). (Methods in Molecular Biology; Vol. 783). https://doi.org/10.1007/978-1-61779-282-3_4

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