Probing the force generation and stepping behavior of cytoplasmic dynein

Arne Gennerich, Samara L. Reck-Peterson

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Cytoplasmic dynein, which is the largest and arguably the most complex cytoskeletal motor protein, plays fundamental roles during cell division, nuclear positioning, and organelle and mRNA transport, by generating force and movement toward the minus ends of microtubules. Consequently, dynein is central to many physiological processes, and its dysfunction is implicated in human diseases. However, the molecular mechanism by which dynein produces force and movement remains poorly understood. Here, we describe the use of optical tweezers to probe the nanometer-scale motion and force generation of individual dynein molecules, and provide a hands-on protocol for how to purify cytoplasmic dynein from budding yeast in amounts sufficient for single-molecule studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages63-80
Number of pages18
Volume783
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume783
ISSN (Print)10643745

Fingerprint

Cytoplasmic Dyneins
Dyneins
Optical Tweezers
Physiological Phenomena
Saccharomycetales
Cytoskeletal Proteins
Microtubules
Organelles
Cell Division
Messenger RNA

Keywords

  • Budding yeast
  • Cytoplasmic dynein
  • Microtubules
  • Molecular motors
  • Optical trapping
  • Optical tweezers
  • Saccharomyces cerevisiae
  • Single-molecule assays

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gennerich, A., & Reck-Peterson, S. L. (2011). Probing the force generation and stepping behavior of cytoplasmic dynein. In Methods in Molecular Biology (Vol. 783, pp. 63-80). (Methods in Molecular Biology; Vol. 783). https://doi.org/10.1007/978-1-61779-282-3_4

Probing the force generation and stepping behavior of cytoplasmic dynein. / Gennerich, Arne; Reck-Peterson, Samara L.

Methods in Molecular Biology. Vol. 783 2011. p. 63-80 (Methods in Molecular Biology; Vol. 783).

Research output: Chapter in Book/Report/Conference proceedingChapter

Gennerich, A & Reck-Peterson, SL 2011, Probing the force generation and stepping behavior of cytoplasmic dynein. in Methods in Molecular Biology. vol. 783, Methods in Molecular Biology, vol. 783, pp. 63-80. https://doi.org/10.1007/978-1-61779-282-3_4
Gennerich A, Reck-Peterson SL. Probing the force generation and stepping behavior of cytoplasmic dynein. In Methods in Molecular Biology. Vol. 783. 2011. p. 63-80. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-282-3_4
Gennerich, Arne ; Reck-Peterson, Samara L. / Probing the force generation and stepping behavior of cytoplasmic dynein. Methods in Molecular Biology. Vol. 783 2011. pp. 63-80 (Methods in Molecular Biology).
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