Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry

Jing Qu Guan, Steven C. Almo, Mark R. Chance

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The conformational differences between Ca 2+-ATP--actin and Mg 2+-ATP-G-actin probed by hydroxyl radicals and mass spectrometry (MS) were analyzed. Hydroxyl radicals generated by synchrotron x-rays probed the solvent accessibilities of discrete residues of a protein by forming a covalent adduct with a side chain. The solvent accessibilities were measured in terms of modification rates quantitated by MS, and the modified sites were identified by MS/MS. The conformational change of the protein was found to be correlated with the solvent accessibility variation of residues.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages275-276
Number of pages2
StatePublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Hydroxyl Radical
Mass spectrometry
Conformations
Synchrotrons
Proteins
X rays
ATP-G-actin

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Guan, J. Q., Almo, S. C., & Chance, M. R. (2002). Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 275-276)

Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry. / Guan, Jing Qu; Almo, Steven C.; Chance, Mark R.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 275-276.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Guan, JQ, Almo, SC & Chance, MR 2002, Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 275-276, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Guan JQ, Almo SC, Chance MR. Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 275-276
Guan, Jing Qu ; Almo, Steven C. ; Chance, Mark R. / Probing the conformation difference between Ca 2+-ATP-actin and Mg 2+-ATP-actin by hydroxyl radicals and mass spectrometry. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 275-276
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