Proapoptotic function of protein kinase CK2α″ is mediated by a JNK signaling cascade

Philip Hilgard, Mark J. Czaja, Guido Gerken, Richard J. Stockert

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Protein kinase CK2 (formerly casein kinase II) is a tetrameric enzyme constitutively expressed in all eurakyotic tissues that plays a significant role in the regulation of cell proliferation, malignant transformation, and apoptosis. The catalytic α-subunit of the enzyme is known to exist in three isoforms CK2α, CK2α and CK2α". CK2α" is highly expressed in liver compared with other tissues and is required for the normal trafficking of several hepatocellular membrane proteins. Initial studies of dengue virus infection indicated that the CK2α"-deficient membrane trafficking mutant cell line (Trfl) was resistant to virus-induced cell death compared with the parental human hepatoma (HuH)-7 hepatoma line. Expression of recombinant CK2α" in Trf1 was capable of reverting this resistant phenotype. This study was extended to TNF-α in addition to other stimuli of cell death in an attempt to uncover common death pathways that might be modulated by CK2α". Evaluation of different pathways involved in death signaling suggest that the regulation of a critical proapoptotic step in HuH-7 cells by CK2α" is mediated by a JNK signaling cascade.

Original languageEnglish (US)
Pages (from-to)G192-G201
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume287
Issue number1 50-1
DOIs
StatePublished - Jul 1 2004

Keywords

  • Activator protein-1
  • Casein kinase
  • Dengue virus
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

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