Priming of protective Anti-Listeria monocytogenes memory CD8+ T cells requires a functional SecA2 secretion system

Massilva Rahmoun, Marilyn Gros, Laura Campisi, Delphine Bassand, Anne Lazzari, Christophe Massiera, Emilie Narni-Mancinelli, Pierre Gounon, Gregoire Lauvau

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The SecA2 auxiliary secretion system of Gram-positive bacteria promotes the export of virulence proteins essential for colonization of the host in the case of both Mycobacterium tuberculosis and Listeria monocytogenes, two intracellular bacteria causing diseases in humans. We and others have demonstrated that this secretion system is also linked to the onset of long-term CD8+ T cell-mediated protective immunity in mice. In the case of L. monocytogenes, expression of SecA2 inside the cytosol of infected cells correlates with the generation of CCL3-secreting memory CD8+ T cells that are required for protection against secondary challenge with wild-type (wt) L. monocytogenes. Since the SecA2 ATPase is well conserved among Gram-positive pathogenic bacteria, we hypothesized that SecA2 itself bears evolutionarily conserved motifs recognized by cytosolic pattern recognition receptors, leading to signaling events promoting the differentiation of CCL3+ memory CD8+ T cells. To test this possibility, we generated a stable L. monocytogenes chromosomal mutant that expressed a SecA2 ATPase bearing a mutated nucleotide binding site (NBS). Similarly to a SecA2 deletion mutant, the NBS mutant exhibited rough colonies, a bacterial chaining phenotype, an impaired protein secretion profile, and in vivo virulence in comparison to wt L. monocytogenes. Importantly, mice immunized with the SecA2 NBS mutant were not protected against secondary infection with wt L. monocytogenes and did not develop CCL3+ memory CD8+ T cells. NBS mutant and wt SecA2 proteins were expressed to comparable extents by bacteria, suggesting that SecA2 itself is unlikely to promote the induction of these cells. Rather, one or several of the SecA2 substrate proteins released inside the cytosol of infected cells may be involved.

Original languageEnglish (US)
Pages (from-to)2396-2403
Number of pages8
JournalInfection and Immunity
Volume79
Issue number6
DOIs
StatePublished - Jun 2011

Fingerprint

Listeria monocytogenes
T-Lymphocytes
Nucleotides
Binding Sites
Gram-Positive Bacteria
Cytosol
Virulence
Adenosine Triphosphatases
Proteins
Bacteria
Pattern Recognition Receptors
Cytoprotection
Coinfection
Mycobacterium tuberculosis
Cellular Immunity
Phenotype

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Priming of protective Anti-Listeria monocytogenes memory CD8+ T cells requires a functional SecA2 secretion system. / Rahmoun, Massilva; Gros, Marilyn; Campisi, Laura; Bassand, Delphine; Lazzari, Anne; Massiera, Christophe; Narni-Mancinelli, Emilie; Gounon, Pierre; Lauvau, Gregoire.

In: Infection and Immunity, Vol. 79, No. 6, 06.2011, p. 2396-2403.

Research output: Contribution to journalArticle

Rahmoun, M, Gros, M, Campisi, L, Bassand, D, Lazzari, A, Massiera, C, Narni-Mancinelli, E, Gounon, P & Lauvau, G 2011, 'Priming of protective Anti-Listeria monocytogenes memory CD8+ T cells requires a functional SecA2 secretion system', Infection and Immunity, vol. 79, no. 6, pp. 2396-2403. https://doi.org/10.1128/IAI.00020-11
Rahmoun, Massilva ; Gros, Marilyn ; Campisi, Laura ; Bassand, Delphine ; Lazzari, Anne ; Massiera, Christophe ; Narni-Mancinelli, Emilie ; Gounon, Pierre ; Lauvau, Gregoire. / Priming of protective Anti-Listeria monocytogenes memory CD8+ T cells requires a functional SecA2 secretion system. In: Infection and Immunity. 2011 ; Vol. 79, No. 6. pp. 2396-2403.
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