Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators

Ulrich G. Steidl, Simone Bork, Sebastian Schaub, Oliver Selbach, Janette Seres, Manuel Alvado, Thomas Schroeder, Ulrich Peter Rohr, Roland Fenk, Slawomir Kliszewski, Christian Maercker, Peter Neubert, Stefan R. Bornstein, Helmut L. Haas, Guido Kobbe, Daniel G. Tenen, Rainer Haas, Ralf Kronenwett

Research output: Contribution to journalArticle

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Abstract

Recently, overlapping molecular phenotypes of hematopoietic and neuropoietic cells were described in mice. Here, we examined primary human CD34+ hematopoietic stem and progenitor cells applying specialized cDNA arrays, real-time reverse-transcriptase-polymerase chain reaction (RT-PCR), and fluorescent-activated cell sorter (FACS) analysis focusing on genes involved in neurobiologic functions. We found expression of vesicle fusion and motility genes, ligand- and voltage-gated ion channels, receptor kinases and phosphatases, and, most interestingly, mRNA as well as protein expression of G protein-coupled receptors of neuromediators (corticotropin-releasing hormone 1 [CRH1] and CRH2 receptors, orexin/hypocretin 1 and 2 receptors, GABA B receptor, adenosine A2B receptor, opioid κ1 and μ1 receptors, and 5-HT 1F receptor). As shown by 2-color immunofluorescence, the protein expression of these receptors was higher in the more immature CD38dim than in the CD38bright subset within the CD34+ population, and completely absent in fully differentiated blood cells, suggesting that those receptors play a role in developmentally early CD34 + stem and progenitor cells. The intracellular concentration of cyclic adenosine monophosphate (cAMP) in CD34+ cells was diminished significantly upon stimulation of either CRH or orexin receptors, indicating that those are functionally active and coupled to inhibitory G proteins in human hematopoietic cells. In conclusion, these findings suggest a molecular interrelation of neuronal and hematopoietic signaling mechanisms in humans.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalBlood
Volume104
Issue number1
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

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Orexin Receptors
Neurotransmitter Receptor
Hematopoietic Stem Cells
Adenosine A2B Receptors
Stem Cells
Genes
GABA-B Receptors
Ligand-Gated Ion Channels
Corticotropin-Releasing Hormone Receptors
Gene Fusion
Corticotropin-Releasing Hormone
Polymerase chain reaction
RNA-Directed DNA Polymerase
G-Protein-Coupled Receptors
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Ion Channels
GTP-Binding Proteins
Phosphoric Monoester Hydrolases
Cyclic AMP

ASJC Scopus subject areas

  • Hematology

Cite this

Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators. / Steidl, Ulrich G.; Bork, Simone; Schaub, Sebastian; Selbach, Oliver; Seres, Janette; Alvado, Manuel; Schroeder, Thomas; Rohr, Ulrich Peter; Fenk, Roland; Kliszewski, Slawomir; Maercker, Christian; Neubert, Peter; Bornstein, Stefan R.; Haas, Helmut L.; Kobbe, Guido; Tenen, Daniel G.; Haas, Rainer; Kronenwett, Ralf.

In: Blood, Vol. 104, No. 1, 01.07.2004, p. 81-88.

Research output: Contribution to journalArticle

Steidl, UG, Bork, S, Schaub, S, Selbach, O, Seres, J, Alvado, M, Schroeder, T, Rohr, UP, Fenk, R, Kliszewski, S, Maercker, C, Neubert, P, Bornstein, SR, Haas, HL, Kobbe, G, Tenen, DG, Haas, R & Kronenwett, R 2004, 'Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators', Blood, vol. 104, no. 1, pp. 81-88. https://doi.org/10.1182/blood-2004-01-0373
Steidl, Ulrich G. ; Bork, Simone ; Schaub, Sebastian ; Selbach, Oliver ; Seres, Janette ; Alvado, Manuel ; Schroeder, Thomas ; Rohr, Ulrich Peter ; Fenk, Roland ; Kliszewski, Slawomir ; Maercker, Christian ; Neubert, Peter ; Bornstein, Stefan R. ; Haas, Helmut L. ; Kobbe, Guido ; Tenen, Daniel G. ; Haas, Rainer ; Kronenwett, Ralf. / Primary human CD34+ hematopoietic stem and progenitor cells express functionally active receptors of neuromediators. In: Blood. 2004 ; Vol. 104, No. 1. pp. 81-88.
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AU - Steidl, Ulrich G.

AU - Bork, Simone

AU - Schaub, Sebastian

AU - Selbach, Oliver

AU - Seres, Janette

AU - Alvado, Manuel

AU - Schroeder, Thomas

AU - Rohr, Ulrich Peter

AU - Fenk, Roland

AU - Kliszewski, Slawomir

AU - Maercker, Christian

AU - Neubert, Peter

AU - Bornstein, Stefan R.

AU - Haas, Helmut L.

AU - Kobbe, Guido

AU - Tenen, Daniel G.

AU - Haas, Rainer

AU - Kronenwett, Ralf

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