TY - JOUR
T1 - Primary fixation in osmium-potassium ferrocyanide
T2 - The staining of glycogen, glycoproteins, elastin, an intranuclear reticular structure, and intercisternal trabeculae
AU - Goldfischer, S.
AU - Kress, Y.
AU - Coltoff-Schiller, B.
AU - Berman, J.
PY - 1981
Y1 - 1981
N2 - Primary fixation in an osmium-potassium ferrocyanide (K4Fe(CN)6) mixture combined selective fixation, staining, and extraction of various cellular components; membranes, glycogen, glycoproteins, and elastin are preserved and stained. An intranuclear reticular structure that is composed of 3-6 nm fibers and permeates the entire nucleus, except for the nuclear pores, is demonstrated by electron microscopic examination of tissues prepared in an osmium-potassium ferrocyanide fixative. Condensations of the reticulum parallel the distribution of heterochromatin in interphase nuclei. This preparative procedure also reveals a network of trabeculae that are associated with the cisternae of rough endoplasmic reticulum and connect the parallel cisternae in hepatocytes, plasmacytes, neurons, and pancreatic acinar cells. The intercisternal trabeculae are associated with both free and bound ribosomes.
AB - Primary fixation in an osmium-potassium ferrocyanide (K4Fe(CN)6) mixture combined selective fixation, staining, and extraction of various cellular components; membranes, glycogen, glycoproteins, and elastin are preserved and stained. An intranuclear reticular structure that is composed of 3-6 nm fibers and permeates the entire nucleus, except for the nuclear pores, is demonstrated by electron microscopic examination of tissues prepared in an osmium-potassium ferrocyanide fixative. Condensations of the reticulum parallel the distribution of heterochromatin in interphase nuclei. This preparative procedure also reveals a network of trabeculae that are associated with the cisternae of rough endoplasmic reticulum and connect the parallel cisternae in hepatocytes, plasmacytes, neurons, and pancreatic acinar cells. The intercisternal trabeculae are associated with both free and bound ribosomes.
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U2 - 10.1177/29.9.6169760
DO - 10.1177/29.9.6169760
M3 - Article
C2 - 6169760
AN - SCOPUS:0019781686
SN - 0022-1554
VL - 29
SP - 1105
EP - 1111
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
IS - 9
ER -