Primary fixation in osmium-potassium ferrocyanide

The staining of glycogen, glycoproteins, elastin, an intranuclear reticular structure, and intercisternal trabeculae

S. Goldfischer, Y. Kress, B. Coltoff-Schiller, Joan W. Berman

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31 Citations (Scopus)

Abstract

Primary fixation in an osmium-potassium ferrocyanide (K4Fe(CN)6) mixture combined selective fixation, staining, and extraction of various cellular components; membranes, glycogen, glycoproteins, and elastin are preserved and stained. An intranuclear reticular structure that is composed of 3-6 nm fibers and permeates the entire nucleus, except for the nuclear pores, is demonstrated by electron microscopic examination of tissues prepared in an osmium-potassium ferrocyanide fixative. Condensations of the reticulum parallel the distribution of heterochromatin in interphase nuclei. This preparative procedure also reveals a network of trabeculae that are associated with the cisternae of rough endoplasmic reticulum and connect the parallel cisternae in hepatocytes, plasmacytes, neurons, and pancreatic acinar cells. The intercisternal trabeculae are associated with both free and bound ribosomes.

Original languageEnglish (US)
Pages (from-to)1105-1111
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume29
Issue number9
StatePublished - 1981

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Osmium
Elastin
Glycogen
Glycoproteins
Staining and Labeling
Nuclear Pore
Fixatives
Reticulum
Heterochromatin
Rough Endoplasmic Reticulum
Acinar Cells
Interphase
Membrane Glycoproteins
Plasma Cells
Ribosomes
Hepatocytes
Electrons
Neurons
potassium ferrocyanide

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

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abstract = "Primary fixation in an osmium-potassium ferrocyanide (K4Fe(CN)6) mixture combined selective fixation, staining, and extraction of various cellular components; membranes, glycogen, glycoproteins, and elastin are preserved and stained. An intranuclear reticular structure that is composed of 3-6 nm fibers and permeates the entire nucleus, except for the nuclear pores, is demonstrated by electron microscopic examination of tissues prepared in an osmium-potassium ferrocyanide fixative. Condensations of the reticulum parallel the distribution of heterochromatin in interphase nuclei. This preparative procedure also reveals a network of trabeculae that are associated with the cisternae of rough endoplasmic reticulum and connect the parallel cisternae in hepatocytes, plasmacytes, neurons, and pancreatic acinar cells. The intercisternal trabeculae are associated with both free and bound ribosomes.",
author = "S. Goldfischer and Y. Kress and B. Coltoff-Schiller and Berman, {Joan W.}",
year = "1981",
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pages = "1105--1111",
journal = "Journal of Histochemistry and Cytochemistry",
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TY - JOUR

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T2 - The staining of glycogen, glycoproteins, elastin, an intranuclear reticular structure, and intercisternal trabeculae

AU - Goldfischer, S.

AU - Kress, Y.

AU - Coltoff-Schiller, B.

AU - Berman, Joan W.

PY - 1981

Y1 - 1981

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