Preterm rabbit model of glycerol-induced intraventricular hemorrhage

Praveen Ballabh

doi:10.1007/978-1-4939-2709-8_4

Preterm rabbit model of glycerol-induced intraventricular hemorrhage

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Intraventricular hemorrhage (IVH) remains a major complication of prematurity. We have developed a model of IVH in preterm rabbit pups. We deliver rabbit pups prematurely by C-section at 29-day gestational age (term = 32 days) and inject intraperitoneal glycerol at 3 h age to induce IVH. About 80 % of glycerol-treated pups develop IVH within 8 h age, which can be detected accurately with head ultrasound. These pups are reared in an infant incubator and gavage fed. At postnatal day 14, they exhibit hypomyelination, gliosis, and impaired neurological function. Hence, this is a novel animal model of IVH that can be used to test strategies to prevent post-hemorrhagic complication of IVH.

Original language	English (US)
Title of host publication	Animal Models of Neurodevelopmental Disorders
Publisher	Springer New York
Pages	45-54
Number of pages	10
ISBN (Electronic)	9781493927098
ISBN (Print)	9781493927081
DOIs	https://doi.org/10.1007/978-1-4939-2709-8_4
State	Published - Sep 19 2015
Externally published	Yes

Keywords

Germinal matrix
Gliosis
Glycerol
Head ultrasound
Hypomyelination
Intraventricular hemorrhage

ASJC Scopus subject areas

General Medicine
General Neuroscience
General Biochemistry, Genetics and Molecular Biology

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10.1007/978-1-4939-2709-8_4

Cite this

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abstract = "Intraventricular hemorrhage (IVH) remains a major complication of prematurity. We have developed a model of IVH in preterm rabbit pups. We deliver rabbit pups prematurely by C-section at 29-day gestational age (term = 32 days) and inject intraperitoneal glycerol at 3 h age to induce IVH. About 80 % of glycerol-treated pups develop IVH within 8 h age, which can be detected accurately with head ultrasound. These pups are reared in an infant incubator and gavage fed. At postnatal day 14, they exhibit hypomyelination, gliosis, and impaired neurological function. Hence, this is a novel animal model of IVH that can be used to test strategies to prevent post-hemorrhagic complication of IVH.",

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AB - Intraventricular hemorrhage (IVH) remains a major complication of prematurity. We have developed a model of IVH in preterm rabbit pups. We deliver rabbit pups prematurely by C-section at 29-day gestational age (term = 32 days) and inject intraperitoneal glycerol at 3 h age to induce IVH. About 80 % of glycerol-treated pups develop IVH within 8 h age, which can be detected accurately with head ultrasound. These pups are reared in an infant incubator and gavage fed. At postnatal day 14, they exhibit hypomyelination, gliosis, and impaired neurological function. Hence, this is a novel animal model of IVH that can be used to test strategies to prevent post-hemorrhagic complication of IVH.

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KW - Gliosis

KW - Glycerol

KW - Head ultrasound

KW - Hypomyelination

KW - Intraventricular hemorrhage

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Preterm rabbit model of glycerol-induced intraventricular hemorrhage

Abstract

Keywords

ASJC Scopus subject areas

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