Pressure volume curves in arrested heterotopic rat heart isografts

Role of improved myocardial protection

Joanne P. Starr, Chao Xiang Jia, David G. Rabkin, Mehrdad M R Amirhamzeh, Joseph P. Hart, Daphne T. Hsu, Pablo Soto, David Pinsky, Henry M. Spotnitz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background. To minimize decreases in left ventricular (LV) compliance immediately after rat heart transplantation, we tested several different methods of myocardial protection. Materials and methods. Five groups of ACI rat hearts (n = 6 each) were arrested by coronary perfusion with 5 ml of UW (University of Wisconsin), UW-BDM (UW with 2,3-butanedione monoxime), CU (Columbia University), or CU-BDM solution or by LV injection of potassium chloride and Ringer's lactate immersion (KCI/RL). After abdominal isografting and blood reperfusion for 15 min, transplanted hearts (TxH) were arrested and excised. Diastolic LV pressure-volume curves (LVPVCs) were correlated with myocardial water content (MWC). Native hearts (NH) were arrested identically to TxH and maintained at 4°C by immersion. LVPVCs were measured at 15min intervals for 90 min. Results. In three of four pressure intervals at Time 0, normalized LV volume (LVV) was smaller (P < 0.05, ANOVA) in KCI/RL native hearts than in the four perfusion groups. LVV decreased significantly in NH after 45-75 min; LVV decreased similarly with time in all groups. In TxH, postarrest LVVs were higher with UWBDM, CU-BDM, and CU than with UW or KCl/RL (P < 0.05, ANOVA). Expressing LVV of TxH as a percentage of NH, UW-BDM, CU, and CU-BDM provided qualitatively better diastolic properties than KCl/RL and UW. Conclusions. Thus rat LVPVCs can be improved after heart transplantation with alternative strategies of myocardial protection. KCl arrest decreases LV filling volume in this model and should be avoided.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalJournal of Surgical Research
Volume86
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Isografts
Pressure
Immersion
Heart Transplantation
Analysis of Variance
Perfusion
Inbred ACI Rats
Isogeneic Transplantation
Potassium Chloride
Ventricular Pressure
Compliance
Reperfusion
Injections
Water

Keywords

  • Coronary perfusates
  • Diastolic properties
  • Myocardial protection
  • Ventricular compliance

ASJC Scopus subject areas

  • Surgery

Cite this

Starr, J. P., Jia, C. X., Rabkin, D. G., Amirhamzeh, M. M. R., Hart, J. P., Hsu, D. T., ... Spotnitz, H. M. (1999). Pressure volume curves in arrested heterotopic rat heart isografts: Role of improved myocardial protection. Journal of Surgical Research, 86(1), 123-129. https://doi.org/10.1006/jsre.1999.5683

Pressure volume curves in arrested heterotopic rat heart isografts : Role of improved myocardial protection. / Starr, Joanne P.; Jia, Chao Xiang; Rabkin, David G.; Amirhamzeh, Mehrdad M R; Hart, Joseph P.; Hsu, Daphne T.; Soto, Pablo; Pinsky, David; Spotnitz, Henry M.

In: Journal of Surgical Research, Vol. 86, No. 1, 1999, p. 123-129.

Research output: Contribution to journalArticle

Starr, JP, Jia, CX, Rabkin, DG, Amirhamzeh, MMR, Hart, JP, Hsu, DT, Soto, P, Pinsky, D & Spotnitz, HM 1999, 'Pressure volume curves in arrested heterotopic rat heart isografts: Role of improved myocardial protection', Journal of Surgical Research, vol. 86, no. 1, pp. 123-129. https://doi.org/10.1006/jsre.1999.5683
Starr, Joanne P. ; Jia, Chao Xiang ; Rabkin, David G. ; Amirhamzeh, Mehrdad M R ; Hart, Joseph P. ; Hsu, Daphne T. ; Soto, Pablo ; Pinsky, David ; Spotnitz, Henry M. / Pressure volume curves in arrested heterotopic rat heart isografts : Role of improved myocardial protection. In: Journal of Surgical Research. 1999 ; Vol. 86, No. 1. pp. 123-129.
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abstract = "Background. To minimize decreases in left ventricular (LV) compliance immediately after rat heart transplantation, we tested several different methods of myocardial protection. Materials and methods. Five groups of ACI rat hearts (n = 6 each) were arrested by coronary perfusion with 5 ml of UW (University of Wisconsin), UW-BDM (UW with 2,3-butanedione monoxime), CU (Columbia University), or CU-BDM solution or by LV injection of potassium chloride and Ringer's lactate immersion (KCI/RL). After abdominal isografting and blood reperfusion for 15 min, transplanted hearts (TxH) were arrested and excised. Diastolic LV pressure-volume curves (LVPVCs) were correlated with myocardial water content (MWC). Native hearts (NH) were arrested identically to TxH and maintained at 4°C by immersion. LVPVCs were measured at 15min intervals for 90 min. Results. In three of four pressure intervals at Time 0, normalized LV volume (LVV) was smaller (P < 0.05, ANOVA) in KCI/RL native hearts than in the four perfusion groups. LVV decreased significantly in NH after 45-75 min; LVV decreased similarly with time in all groups. In TxH, postarrest LVVs were higher with UWBDM, CU-BDM, and CU than with UW or KCl/RL (P < 0.05, ANOVA). Expressing LVV of TxH as a percentage of NH, UW-BDM, CU, and CU-BDM provided qualitatively better diastolic properties than KCl/RL and UW. Conclusions. Thus rat LVPVCs can be improved after heart transplantation with alternative strategies of myocardial protection. KCl arrest decreases LV filling volume in this model and should be avoided.",
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T1 - Pressure volume curves in arrested heterotopic rat heart isografts

T2 - Role of improved myocardial protection

AU - Starr, Joanne P.

AU - Jia, Chao Xiang

AU - Rabkin, David G.

AU - Amirhamzeh, Mehrdad M R

AU - Hart, Joseph P.

AU - Hsu, Daphne T.

AU - Soto, Pablo

AU - Pinsky, David

AU - Spotnitz, Henry M.

PY - 1999

Y1 - 1999

N2 - Background. To minimize decreases in left ventricular (LV) compliance immediately after rat heart transplantation, we tested several different methods of myocardial protection. Materials and methods. Five groups of ACI rat hearts (n = 6 each) were arrested by coronary perfusion with 5 ml of UW (University of Wisconsin), UW-BDM (UW with 2,3-butanedione monoxime), CU (Columbia University), or CU-BDM solution or by LV injection of potassium chloride and Ringer's lactate immersion (KCI/RL). After abdominal isografting and blood reperfusion for 15 min, transplanted hearts (TxH) were arrested and excised. Diastolic LV pressure-volume curves (LVPVCs) were correlated with myocardial water content (MWC). Native hearts (NH) were arrested identically to TxH and maintained at 4°C by immersion. LVPVCs were measured at 15min intervals for 90 min. Results. In three of four pressure intervals at Time 0, normalized LV volume (LVV) was smaller (P < 0.05, ANOVA) in KCI/RL native hearts than in the four perfusion groups. LVV decreased significantly in NH after 45-75 min; LVV decreased similarly with time in all groups. In TxH, postarrest LVVs were higher with UWBDM, CU-BDM, and CU than with UW or KCl/RL (P < 0.05, ANOVA). Expressing LVV of TxH as a percentage of NH, UW-BDM, CU, and CU-BDM provided qualitatively better diastolic properties than KCl/RL and UW. Conclusions. Thus rat LVPVCs can be improved after heart transplantation with alternative strategies of myocardial protection. KCl arrest decreases LV filling volume in this model and should be avoided.

AB - Background. To minimize decreases in left ventricular (LV) compliance immediately after rat heart transplantation, we tested several different methods of myocardial protection. Materials and methods. Five groups of ACI rat hearts (n = 6 each) were arrested by coronary perfusion with 5 ml of UW (University of Wisconsin), UW-BDM (UW with 2,3-butanedione monoxime), CU (Columbia University), or CU-BDM solution or by LV injection of potassium chloride and Ringer's lactate immersion (KCI/RL). After abdominal isografting and blood reperfusion for 15 min, transplanted hearts (TxH) were arrested and excised. Diastolic LV pressure-volume curves (LVPVCs) were correlated with myocardial water content (MWC). Native hearts (NH) were arrested identically to TxH and maintained at 4°C by immersion. LVPVCs were measured at 15min intervals for 90 min. Results. In three of four pressure intervals at Time 0, normalized LV volume (LVV) was smaller (P < 0.05, ANOVA) in KCI/RL native hearts than in the four perfusion groups. LVV decreased significantly in NH after 45-75 min; LVV decreased similarly with time in all groups. In TxH, postarrest LVVs were higher with UWBDM, CU-BDM, and CU than with UW or KCl/RL (P < 0.05, ANOVA). Expressing LVV of TxH as a percentage of NH, UW-BDM, CU, and CU-BDM provided qualitatively better diastolic properties than KCl/RL and UW. Conclusions. Thus rat LVPVCs can be improved after heart transplantation with alternative strategies of myocardial protection. KCl arrest decreases LV filling volume in this model and should be avoided.

KW - Coronary perfusates

KW - Diastolic properties

KW - Myocardial protection

KW - Ventricular compliance

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