TY - JOUR
T1 - Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins
AU - Hogue‐Angeletti, R. A.
AU - Wu, H. ‐L
AU - Schlaepfer, W. W.
PY - 1982/1
Y1 - 1982/1
N2 - Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.
AB - Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.
KW - Amino acid composition
KW - Neurofilament triplet proteins
KW - Neurofilaments
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U2 - 10.1111/j.1471-4159.1982.tb10861.x
DO - 10.1111/j.1471-4159.1982.tb10861.x
M3 - Article
C2 - 6809894
AN - SCOPUS:0020075125
SN - 0022-3042
VL - 38
SP - 116
EP - 120
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -