Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins

R. A. Hogue‐Angeletti; H. ‐L Wu; W. W. Schlaepfer

doi:10.1111/j.1471-4159.1982.tb10861.x

Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins

R. A. Hogue‐Angeletti, H. ‐L Wu, W. W. Schlaepfer

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.

Original language	English (US)
Pages (from-to)	116-120
Number of pages	5
Journal	Journal of Neurochemistry
Volume	38
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-4159.1982.tb10861.x
State	Published - Jan 1982
Externally published	Yes

Keywords

Amino acid composition
Neurofilament triplet proteins
Neurofilaments

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

Access to Document

10.1111/j.1471-4159.1982.tb10861.x

Cite this

@article{dd2c1d8612924a278b6d79620101eb94,

title = "Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins",

abstract = "Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.",

keywords = "Amino acid composition, Neurofilament triplet proteins, Neurofilaments",

author = "Hogue‐Angeletti, {R. A.} and Wu, {H. ‐L} and Schlaepfer, {W. W.}",

year = "1982",

month = jan,

doi = "10.1111/j.1471-4159.1982.tb10861.x",

language = "English (US)",

volume = "38",

pages = "116--120",

journal = "Journal of Neurochemistry",

issn = "0022-3042",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins

AU - Hogue‐Angeletti, R. A.

AU - Wu, H. ‐L

AU - Schlaepfer, W. W.

PY - 1982/1

Y1 - 1982/1

N2 - Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.

AB - Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.

KW - Amino acid composition

KW - Neurofilament triplet proteins

KW - Neurofilaments

UR - http://www.scopus.com/inward/record.url?scp=0020075125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020075125&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1982.tb10861.x

DO - 10.1111/j.1471-4159.1982.tb10861.x

M3 - Article

C2 - 6809894

AN - SCOPUS:0020075125

SN - 0022-3042

VL - 38

SP - 116

EP - 120

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

IS - 1

ER -

Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this