Preparative Separation and Amino Acid Composition of Neurofilament Triplet Proteins

R. A. Hogue‐Angeletti, H. ‐L Wu, W. W. Schlaepfer

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Abstract: Intact neurofilaments were isolated from bovine spinal cord white matter, washed by sedimentation in 0.1 m‐NaCl, and extracted with 8 m‐urea. Solubilized neurofilament triplet proteins of molecular weights approximately 68,000 (P68), 150,000 (P150), and 200,000 (P200) were purified by preparative electrophoresis, using an LKB 7900 Uniphor apparatus. The method provides for an enhanced yield of purified protein and has markedly reduced admixture of electrophoresed protein with acrylamide and associated protein contaminants. Amino acid compositions of the purified neurofilament triplet proteins are reported and compared.

Original languageEnglish (US)
Pages (from-to)116-120
Number of pages5
JournalJournal of Neurochemistry
Volume38
Issue number1
DOIs
StatePublished - Jan 1982

Keywords

  • Amino acid composition
  • Neurofilament triplet proteins
  • Neurofilaments

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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