TY - JOUR
T1 - Preparation of [35S]sulfobromophthalein of high specific activity
AU - Kurisu, Hiroko
AU - Nilprabhassorn, Polly
AU - Wolkoff, Allan W.
N1 - Funding Information:
This work was supported by NIH Grants DK-23026 and DK-41296.
PY - 1989/5/15
Y1 - 1989/5/15
N2 - Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. [3H]Bilirubin and [35S]sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare [35S]sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.
AB - Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. [3H]Bilirubin and [35S]sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare [35S]sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.
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U2 - 10.1016/0003-2697(89)90202-9
DO - 10.1016/0003-2697(89)90202-9
M3 - Article
C2 - 2757201
AN - SCOPUS:0024472814
SN - 0003-2697
VL - 179
SP - 72
EP - 74
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -