Preparation of [35S]sulfobromophthalein of high specific activity

Hiroko Kurisu, Polly Nilprabhassorn, Allan W. Wolkoff

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Study of the hepatocyte transport mechanism of organic anions such as bilirubin and sulfobromophthalein has been limited by the relatively low specific activities of these ligands. [3H]Bilirubin and [35S]sulfobromophthalein have been available with specific activities of only approximately 100 mCi/mmol. We now report a relatively simple procedure to prepare [35S]sulfobromophthalein at a specific activity of approximately 3000 mCi/mmol. This compound is radiochemically pure and serves as a tracer for authentic sulfobromophthalein as judged by chromatography, hepatocyte uptake, metabolism, and biliary excretion. Use of this material as a photoaffinity probe and as a transported ligand may permit dissection and understanding of its transport mechanism.

Original languageEnglish (US)
Pages (from-to)72-74
Number of pages3
JournalAnalytical Biochemistry
Volume179
Issue number1
DOIs
StatePublished - May 15 1989

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Sulfobromophthalein
Bilirubin
Hepatocytes
Ligands
Dissection
Chromatography
Metabolism
Anions

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Preparation of [35S]sulfobromophthalein of high specific activity. / Kurisu, Hiroko; Nilprabhassorn, Polly; Wolkoff, Allan W.

In: Analytical Biochemistry, Vol. 179, No. 1, 15.05.1989, p. 72-74.

Research output: Contribution to journalArticle

Kurisu, Hiroko ; Nilprabhassorn, Polly ; Wolkoff, Allan W. / Preparation of [35S]sulfobromophthalein of high specific activity. In: Analytical Biochemistry. 1989 ; Vol. 179, No. 1. pp. 72-74.
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