Preparation and properties of the brain specific protein 14-3-2

Alfonso Grasso, Giorgio Roda, Ruth A. Hogue-Angeletti, Blake W. Moore, Vernon J. Perez

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A brain specific protein, 14-3-2, has been isolated from bovine brain by the use of ammonium sulfate precipitation, gel filtration on Sephadex G-150, and ion exchange chromatography on DEAE-cellulose and DEAE-Sephadex. It is an acidic protein, in agreement with the high content of glutamic and aspartic acid, that is composed of a single polypeptide chain of mol. wt. 50,000. Immunochemical tests using antiserum to purified 14-3-2 showed that the protein is present in at least 100-fold greater amounts in brain than in any other rat tissue. Furthermore, 14-3-2 was found in brains of a number of vertebrate species, although the antigen is apparently not entirely identical in all species tested. The protein 14-3-2 can be considered to be a species non-specific protein which is neuronal in origin.

Original languageEnglish (US)
Pages (from-to)497-507
Number of pages11
JournalBrain Research
Volume124
Issue number3
DOIs
StatePublished - Apr 1 1977
Externally publishedYes

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Brain
Proteins
DEAE-Dextran
DEAE-Cellulose
Ion Exchange Chromatography
Ammonium Sulfate
Gel Chromatography
Vertebrates
Immune Sera
Glutamic Acid
Antigens
Peptides
sephadex

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Grasso, A., Roda, G., Hogue-Angeletti, R. A., Moore, B. W., & Perez, V. J. (1977). Preparation and properties of the brain specific protein 14-3-2. Brain Research, 124(3), 497-507. https://doi.org/10.1016/0006-8993(77)90949-0

Preparation and properties of the brain specific protein 14-3-2. / Grasso, Alfonso; Roda, Giorgio; Hogue-Angeletti, Ruth A.; Moore, Blake W.; Perez, Vernon J.

In: Brain Research, Vol. 124, No. 3, 01.04.1977, p. 497-507.

Research output: Contribution to journalArticle

Grasso, A, Roda, G, Hogue-Angeletti, RA, Moore, BW & Perez, VJ 1977, 'Preparation and properties of the brain specific protein 14-3-2', Brain Research, vol. 124, no. 3, pp. 497-507. https://doi.org/10.1016/0006-8993(77)90949-0
Grasso A, Roda G, Hogue-Angeletti RA, Moore BW, Perez VJ. Preparation and properties of the brain specific protein 14-3-2. Brain Research. 1977 Apr 1;124(3):497-507. https://doi.org/10.1016/0006-8993(77)90949-0
Grasso, Alfonso ; Roda, Giorgio ; Hogue-Angeletti, Ruth A. ; Moore, Blake W. ; Perez, Vernon J. / Preparation and properties of the brain specific protein 14-3-2. In: Brain Research. 1977 ; Vol. 124, No. 3. pp. 497-507.
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