TY - JOUR
T1 - Preparation and properties of the brain specific protein 14-3-2
AU - Grasso, Alfonso
AU - Roda, Giorgio
AU - Hogue-Angeletti, Ruth A.
AU - Moore, Blake W.
AU - Perez, Vernon J.
N1 - Funding Information:
* Present address: Laboratory of Cell Biology, Rome, Italy. ** Present address: lstituto Superiore di Sanitfi, Rome, Italy. *** Present address: Division of Neuropathology, University of Pennsylvania, School of Medicine, Philadelphia, Pa. 19174, U.S.A. Supported by research grants from the National Institutes of Health NS-05270 and MH-07081.
PY - 1977/4/1
Y1 - 1977/4/1
N2 - A brain specific protein, 14-3-2, has been isolated from bovine brain by the use of ammonium sulfate precipitation, gel filtration on Sephadex G-150, and ion exchange chromatography on DEAE-cellulose and DEAE-Sephadex. It is an acidic protein, in agreement with the high content of glutamic and aspartic acid, that is composed of a single polypeptide chain of mol. wt. 50,000. Immunochemical tests using antiserum to purified 14-3-2 showed that the protein is present in at least 100-fold greater amounts in brain than in any other rat tissue. Furthermore, 14-3-2 was found in brains of a number of vertebrate species, although the antigen is apparently not entirely identical in all species tested. The protein 14-3-2 can be considered to be a species non-specific protein which is neuronal in origin.
AB - A brain specific protein, 14-3-2, has been isolated from bovine brain by the use of ammonium sulfate precipitation, gel filtration on Sephadex G-150, and ion exchange chromatography on DEAE-cellulose and DEAE-Sephadex. It is an acidic protein, in agreement with the high content of glutamic and aspartic acid, that is composed of a single polypeptide chain of mol. wt. 50,000. Immunochemical tests using antiserum to purified 14-3-2 showed that the protein is present in at least 100-fold greater amounts in brain than in any other rat tissue. Furthermore, 14-3-2 was found in brains of a number of vertebrate species, although the antigen is apparently not entirely identical in all species tested. The protein 14-3-2 can be considered to be a species non-specific protein which is neuronal in origin.
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U2 - 10.1016/0006-8993(77)90949-0
DO - 10.1016/0006-8993(77)90949-0
M3 - Article
C2 - 403997
AN - SCOPUS:0017352996
SN - 0006-8993
VL - 124
SP - 497
EP - 507
JO - Brain research
JF - Brain research
IS - 3
ER -