Preparation and properties of the brain specific protein 14-3-2

Alfonso Grasso, Giorgio Roda, Ruth A. Hogue-Angeletti, Blake W. Moore, Vernon J. Perez

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


A brain specific protein, 14-3-2, has been isolated from bovine brain by the use of ammonium sulfate precipitation, gel filtration on Sephadex G-150, and ion exchange chromatography on DEAE-cellulose and DEAE-Sephadex. It is an acidic protein, in agreement with the high content of glutamic and aspartic acid, that is composed of a single polypeptide chain of mol. wt. 50,000. Immunochemical tests using antiserum to purified 14-3-2 showed that the protein is present in at least 100-fold greater amounts in brain than in any other rat tissue. Furthermore, 14-3-2 was found in brains of a number of vertebrate species, although the antigen is apparently not entirely identical in all species tested. The protein 14-3-2 can be considered to be a species non-specific protein which is neuronal in origin.

Original languageEnglish (US)
Pages (from-to)497-507
Number of pages11
JournalBrain research
Issue number3
StatePublished - Apr 1 1977

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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