Preparation and partial fractionation of nonhistone chromosomal proteins from human diploid fibroblasts

Leonard H. Augenlicht, Renato Baserga

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

A method is presented for the isolation of nonhistone chromosomal proteins from chromatin of human diploid WI-38 fibroblasts which involves dissociation of total chromosomal protein from DNA followed by separation into a histone and a nonhistone fraction. Loss of nonhistone protein is minimized at each step, and the only substantial loss, during chromatin dissociation, is shown to be random. Finally, the nonhistone chromosomal protein fraction is separated into five major fractions by column chromatography on QAE Sephadex.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume158
Issue number1
DOIs
StatePublished - Sep 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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