TY - JOUR
T1 - Preparation and partial fractionation of nonhistone chromosomal proteins from human diploid fibroblasts
AU - Augenlicht, Leonard H.
AU - Baserga, Renato
N1 - Funding Information:
1 This work was supported Health Service Research Grant Public Health Service Training CA-05222, and Damon Runyon Grant 1019C.
PY - 1973/9
Y1 - 1973/9
N2 - A method is presented for the isolation of nonhistone chromosomal proteins from chromatin of human diploid WI-38 fibroblasts which involves dissociation of total chromosomal protein from DNA followed by separation into a histone and a nonhistone fraction. Loss of nonhistone protein is minimized at each step, and the only substantial loss, during chromatin dissociation, is shown to be random. Finally, the nonhistone chromosomal protein fraction is separated into five major fractions by column chromatography on QAE Sephadex.
AB - A method is presented for the isolation of nonhistone chromosomal proteins from chromatin of human diploid WI-38 fibroblasts which involves dissociation of total chromosomal protein from DNA followed by separation into a histone and a nonhistone fraction. Loss of nonhistone protein is minimized at each step, and the only substantial loss, during chromatin dissociation, is shown to be random. Finally, the nonhistone chromosomal protein fraction is separated into five major fractions by column chromatography on QAE Sephadex.
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U2 - 10.1016/0003-9861(73)90600-0
DO - 10.1016/0003-9861(73)90600-0
M3 - Article
C2 - 4199638
AN - SCOPUS:0015887085
SN - 0003-9861
VL - 158
SP - 89
EP - 96
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -