Preparation and characterization of Ca2+-Zn2+-derivatives of lentil and pea lectins and comparison with the native forms

Lokesh Bhattacharyya; C. Fred Brewer; Rodney D. Brown; Seymour H. Koenig

doi:10.1016/0006-291X(84)91036-2

Preparation and characterization of Ca²⁺-Zn²⁺-derivatives of lentil and pea lectins and comparison with the native forms

Lokesh Bhattacharyya, C. Fred Brewer, Rodney D. Brown, Seymour H. Koenig

Molecular Pharmacology

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Abstract

Ca²⁺-Zn²⁺-derivatives of lentil and pea lectins were prepared for the first time by a unique method involving dialysis of the native Ca²⁺-Mn²⁺-lectins against large excesses of metal ions in pH 4.0 buffer. Each derivative contained about 1.5 g atoms of Ca²⁺ and about 1 g atom of Zn²⁺ per monomer. The derivatives were found to be identical to their respective native forms, both in molecular weight and carbohydrate binding activities. Solvent proton relaxation dispersion measurements were used to characterize both the Ca²⁺-Zn²⁺- and Ca²⁺-Mn²⁺-complexes of the lentil lectin.

Original language	English (US)
Pages (from-to)	857-862
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	124
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(84)91036-2
State	Published - Nov 14 1984

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Preparation and characterization of Ca2+-Zn2+-derivatives of lentil and pea lectins and comparison with the native forms",

abstract = "Ca2+-Zn2+-derivatives of lentil and pea lectins were prepared for the first time by a unique method involving dialysis of the native Ca2+-Mn2+-lectins against large excesses of metal ions in pH 4.0 buffer. Each derivative contained about 1.5 g atoms of Ca2+ and about 1 g atom of Zn2+ per monomer. The derivatives were found to be identical to their respective native forms, both in molecular weight and carbohydrate binding activities. Solvent proton relaxation dispersion measurements were used to characterize both the Ca2+-Zn2+- and Ca2+-Mn2+-complexes of the lentil lectin.",

author = "Lokesh Bhattacharyya and Brewer, {C. Fred} and Brown, {Rodney D.} and Koenig, {Seymour H.}",

note = "Funding Information: The authors wish to thank Mr. Bert Olson of IBM T. J. Watson Research Center for the atomic absorption analysis. The present work was supported in part by Grant CA 16054, awarded to C.F.B. by the National Cancer Institute, Department of Health, Education and Welfare, and NIH/NCI Core Grant P30 CA 13330.",

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AU - Bhattacharyya, Lokesh

AU - Brewer, C. Fred

AU - Brown, Rodney D.

AU - Koenig, Seymour H.

N1 - Funding Information: The authors wish to thank Mr. Bert Olson of IBM T. J. Watson Research Center for the atomic absorption analysis. The present work was supported in part by Grant CA 16054, awarded to C.F.B. by the National Cancer Institute, Department of Health, Education and Welfare, and NIH/NCI Core Grant P30 CA 13330.

PY - 1984/11/14

Y1 - 1984/11/14

N2 - Ca2+-Zn2+-derivatives of lentil and pea lectins were prepared for the first time by a unique method involving dialysis of the native Ca2+-Mn2+-lectins against large excesses of metal ions in pH 4.0 buffer. Each derivative contained about 1.5 g atoms of Ca2+ and about 1 g atom of Zn2+ per monomer. The derivatives were found to be identical to their respective native forms, both in molecular weight and carbohydrate binding activities. Solvent proton relaxation dispersion measurements were used to characterize both the Ca2+-Zn2+- and Ca2+-Mn2+-complexes of the lentil lectin.

AB - Ca2+-Zn2+-derivatives of lentil and pea lectins were prepared for the first time by a unique method involving dialysis of the native Ca2+-Mn2+-lectins against large excesses of metal ions in pH 4.0 buffer. Each derivative contained about 1.5 g atoms of Ca2+ and about 1 g atom of Zn2+ per monomer. The derivatives were found to be identical to their respective native forms, both in molecular weight and carbohydrate binding activities. Solvent proton relaxation dispersion measurements were used to characterize both the Ca2+-Zn2+- and Ca2+-Mn2+-complexes of the lentil lectin.

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Preparation and characterization of Ca²⁺-Zn²⁺-derivatives of lentil and pea lectins and comparison with the native forms

Abstract

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