Preparation and characterization of Ca2+-Zn2+-derivatives of lentil and pea lectins and comparison with the native forms

Lokesh Bhattacharyya, C. Fred Brewer, Rodney D. Brown, Seymour H. Koenig

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Ca2+-Zn2+-derivatives of lentil and pea lectins were prepared for the first time by a unique method involving dialysis of the native Ca2+-Mn2+-lectins against large excesses of metal ions in pH 4.0 buffer. Each derivative contained about 1.5 g atoms of Ca2+ and about 1 g atom of Zn2+ per monomer. The derivatives were found to be identical to their respective native forms, both in molecular weight and carbohydrate binding activities. Solvent proton relaxation dispersion measurements were used to characterize both the Ca2+-Zn2+- and Ca2+-Mn2+-complexes of the lentil lectin.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume124
Issue number3
DOIs
StatePublished - Nov 14 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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