Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells

Xian Fei Ding, Mou Sun, Fangxia Guan, Li Na Guo, Yan Yan Zhang, You Dong Wan, Xiao Juan Zhang, Yan Wu Yu, Shan Shan Ma, Hai Mu Yao, Rui Yao, Rui Fang Zhang, Tong Wen Sun, Quan Cheng Kan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.

Original languageEnglish (US)
Pages (from-to)1211-1219
Number of pages9
JournalAmerican Journal of Hypertension
Volume30
Issue number12
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Lipopolysaccharides
Adipose Tissue
Renin-Angiotensin System
Blood Pressure
Kidney
Hypertension
Pregnancy
Control Groups
Renin
Monocytes
Lymphocytes

Keywords

  • Blood pressure
  • Hypertension
  • Lipopolysaccharide
  • Offspring
  • Prenatal
  • RAS
  • Stem cells

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells. / Ding, Xian Fei; Sun, Mou; Guan, Fangxia; Guo, Li Na; Zhang, Yan Yan; Wan, You Dong; Zhang, Xiao Juan; Yu, Yan Wu; Ma, Shan Shan; Yao, Hai Mu; Yao, Rui; Zhang, Rui Fang; Sun, Tong Wen; Kan, Quan Cheng.

In: American Journal of Hypertension, Vol. 30, No. 12, 01.12.2017, p. 1211-1219.

Research output: Contribution to journalArticle

Ding, XF, Sun, M, Guan, F, Guo, LN, Zhang, YY, Wan, YD, Zhang, XJ, Yu, YW, Ma, SS, Yao, HM, Yao, R, Zhang, RF, Sun, TW & Kan, QC 2017, 'Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells', American Journal of Hypertension, vol. 30, no. 12, pp. 1211-1219. https://doi.org/10.1093/ajh/hpx117
Ding, Xian Fei ; Sun, Mou ; Guan, Fangxia ; Guo, Li Na ; Zhang, Yan Yan ; Wan, You Dong ; Zhang, Xiao Juan ; Yu, Yan Wu ; Ma, Shan Shan ; Yao, Hai Mu ; Yao, Rui ; Zhang, Rui Fang ; Sun, Tong Wen ; Kan, Quan Cheng. / Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells. In: American Journal of Hypertension. 2017 ; Vol. 30, No. 12. pp. 1211-1219.
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abstract = "BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.",
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T1 - Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells

AU - Ding, Xian Fei

AU - Sun, Mou

AU - Guan, Fangxia

AU - Guo, Li Na

AU - Zhang, Yan Yan

AU - Wan, You Dong

AU - Zhang, Xiao Juan

AU - Yu, Yan Wu

AU - Ma, Shan Shan

AU - Yao, Hai Mu

AU - Yao, Rui

AU - Zhang, Rui Fang

AU - Sun, Tong Wen

AU - Kan, Quan Cheng

PY - 2017/12/1

Y1 - 2017/12/1

N2 - BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.

AB - BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.

KW - Blood pressure

KW - Hypertension

KW - Lipopolysaccharide

KW - Offspring

KW - Prenatal

KW - RAS

KW - Stem cells

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U2 - 10.1093/ajh/hpx117

DO - 10.1093/ajh/hpx117

M3 - Article

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VL - 30

SP - 1211

EP - 1219

JO - Journal of clinical hypertension

JF - Journal of clinical hypertension

SN - 0895-7061

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