Abstract
BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1211-1219 |
| Number of pages | 9 |
| Journal | American Journal of Hypertension |
| Volume | 30 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2017 |
| Externally published | Yes |
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Keywords
- Blood pressure
- Hypertension
- Lipopolysaccharide
- Offspring
- Prenatal
- RAS
- Stem cells
ASJC Scopus subject areas
- Internal Medicine
Cite this
Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells. / Ding, Xian Fei; Sun, Mou; Guan, Fangxia; Guo, Li Na; Zhang, Yan Yan; Wan, You Dong; Zhang, Xiao Juan; Yu, Yan Wu; Ma, Shan Shan; Yao, Hai Mu; Yao, Rui; Zhang, Rui Fang; Sun, Tong Wen; Kan, Quan Cheng.
In: American Journal of Hypertension, Vol. 30, No. 12, 01.12.2017, p. 1211-1219.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Prenatal exposure to LPS alters the intrarenal ras in offspring, which is ameliorated by adipose tissue-derived mesenchymal stem cells
AU - Ding, Xian Fei
AU - Sun, Mou
AU - Guan, Fangxia
AU - Guo, Li Na
AU - Zhang, Yan Yan
AU - Wan, You Dong
AU - Zhang, Xiao Juan
AU - Yu, Yan Wu
AU - Ma, Shan Shan
AU - Yao, Hai Mu
AU - Yao, Rui
AU - Zhang, Rui Fang
AU - Sun, Tong Wen
AU - Kan, Quan Cheng
PY - 2017/12/1
Y1 - 2017/12/1
N2 - BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.
AB - BACKGROUND Prenatal lipopolysaccharide (LPS) exposure causes hypertension in rat offspring through an unknown mechanism. Here, we investigated the role of the intrarenal renin-angiotensin system (RAS) in hypertension induced by prenatal LPS exposure and also explored whether adipose tissue-derived mesenchymal stem cells (ADSCs) can ameliorate the effects of prenatal LPS exposure in rat offspring. METHODS Sixty-four pregnant rats were randomly divided into 4 groups (n = 16 in each), namely, a control group and an LPS group, which were intraperitoneally injected with vehicle and 0.79 mg/kg LPS, respectively, on the 8th, 10th, and 12th days of gestation; an ADSCs group, which was intravenously injected with 1.8 × 107 ADSCs on the 8th, 10th, and 12th days of gestation; and an LPS + ADSCs group, which received a combination of the treatments administered to the LPS and ADSCs groups. RESULTS Prenatal LPS exposure increased blood pressure, Ang II expression, Ang II-positive, monocyte and lymphocyte, apoptotic cells in the kidney, and induced renal histological changes in offspring; however, the LPS and control groups did not differ significantly with respect to plasma renin activity levels, Ang II levels, or renal function. ADSCs treatment attenuated the blood pressure and also ameliorated the other effects of LPS-treated adult offspring. CONCLUSIONS Prenatal exposure to LPS activates the intrarenal RAS but not the circulating RAS and thus induces increases in blood pressure in adult offspring; however, ADSCs treatment attenuates the blood pressure increases resulting from LPS exposure and also ameliorates the other phenotypic changes induced by LPS treatment by inhibiting intrarenal RAS activation.
KW - Blood pressure
KW - Hypertension
KW - Lipopolysaccharide
KW - Offspring
KW - Prenatal
KW - RAS
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=85042130547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042130547&partnerID=8YFLogxK
U2 - 10.1093/ajh/hpx117
DO - 10.1093/ajh/hpx117
M3 - Article
C2 - 28992100
AN - SCOPUS:85042130547
VL - 30
SP - 1211
EP - 1219
JO - Journal of clinical hypertension
JF - Journal of clinical hypertension
SN - 0895-7061
IS - 12
ER -