Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae

Bogos Agianian, Christine Lesch, Olga Loseva, Mitchell S. Dushay

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Hemolymph coagulation is a first response to injury, impeding infection, and ending bleeding. Little is known about its molecular basis in insects, but clotting factors have been identified in the fruit fly Drosophila melanogaster. Here, we have begun to study coagulation in the aquatic larvae of the malaria vector mosquito Anopheles gambiae using methods developed for Drosophila. A delicate clot was seen by light microscopy, and pullout and proteomic analysis identified phenoloxidase and apolipophorin-I as major candidate clotting factors. Electron microscopic analysis confirmed clot formation and revealed it contains fine molecular sheets, most likely a result of lipophorin assembly. Phenoloxidase appears to be more critical in clot formation in Anopheles than in Drosophila. The Anopheles larval clot thus differs in formation, structure, and composition from the clot in Drosophila, confirming the need to study coagulation in different insect species to learn more about its evolution and adaptation to different lifestyles.

Original languageEnglish (US)
Pages (from-to)879-888
Number of pages10
JournalDevelopmental and Comparative Immunology
Volume31
Issue number9
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Anopheles gambiae
Hemolymph
Drosophila
Larva
Anopheles
Blood Coagulation Factors
Monophenol Monooxygenase
Insects
Drosophila melanogaster
Diptera
Proteomics
Malaria
Life Style
Microscopy
Fruit
Electrons
Hemorrhage
Light
Wounds and Injuries
Infection

Keywords

  • Anopheles gambiae
  • Coagulation
  • Drosophila
  • Insect immunity
  • Lipophorin
  • Phenoloxidase

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae. / Agianian, Bogos; Lesch, Christine; Loseva, Olga; Dushay, Mitchell S.

In: Developmental and Comparative Immunology, Vol. 31, No. 9, 2007, p. 879-888.

Research output: Contribution to journalArticle

Agianian, Bogos ; Lesch, Christine ; Loseva, Olga ; Dushay, Mitchell S. / Preliminary characterization of hemolymph coagulation in Anopheles gambiae larvae. In: Developmental and Comparative Immunology. 2007 ; Vol. 31, No. 9. pp. 879-888.
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