Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat

Asher Bashiri, Hye J. Heo, Danny Ben-Avraham, Moshe Mazor, Temuri Budagov, Francine H. Einstein, Gil Atzmon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Maternal obesity is a significant risk factor for development of both maternal and fetal metabolic complications. Increase in visceral fat and insulin resistance is a metabolic hallmark of pregnancy, yet not much is known how obesity alters adipose cellular function and how this may contribute to pregnancy morbidities. We sought to identify alterations in genome-wide transcription expression in both visceral (omental) and abdominal subcutaneous fat deposits in pregnancy complicated by obesity. Visceral and abdominal subcutaneous fat deposits were collected from normal weight and obese pregnant women (n = 4/group) at the time of scheduled uncomplicated cesarean section. A genome-wide expression array (Affymetrix Human Exon 1.0 st platform), validated by quantitative real-time PCR, was utilized to establish the gene transcript expression profile in both visceral and abdominal subcutaneous fat in normal weight and obese pregnant women. Global alteration in gene expression was identified in pregnancy complicated by obesity. These regions of variations led to identification of indolethylamine N-methyltransferase, tissue factor pathway inhibitor-2, and ephrin type-B receptor 6, not previously associated with fat metabolism during pregnancy. In addition, subcutaneous fat of obese pregnant women demonstrated increased coding protein transcripts associated with apoptosis as compared to lean counterparts. Global alteration of gene expression in adipose tissue may contribute to adverse pregnancy outcomes associated with obesity.

Original languageEnglish (US)
Pages (from-to)695-705
Number of pages11
JournalMolecular Genetics and Genomics
Volume289
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Intra-Abdominal Fat
Subcutaneous Fat
Abdominal Subcutaneous Fat
Obesity
Pregnancy
Pregnant Women
tryptamine N-methyltransferase
Mothers
Eph Family Receptors
Genome
Gene Expression
Weights and Measures
Pregnancy Outcome
Transcriptome
Cesarean Section
Insulin Resistance
Adipose Tissue
Real-Time Polymerase Chain Reaction
Exons
Fats

Keywords

  • Gene expression
  • Obesity
  • Omental
  • Pregnancy
  • Subcutaneous

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Bashiri, A., Heo, H. J., Ben-Avraham, D., Mazor, M., Budagov, T., Einstein, F. H., & Atzmon, G. (2014). Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat. Molecular Genetics and Genomics, 289(4), 695-705. https://doi.org/10.1007/s00438-014-0841-5

Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat. / Bashiri, Asher; Heo, Hye J.; Ben-Avraham, Danny; Mazor, Moshe; Budagov, Temuri; Einstein, Francine H.; Atzmon, Gil.

In: Molecular Genetics and Genomics, Vol. 289, No. 4, 2014, p. 695-705.

Research output: Contribution to journalArticle

Bashiri, A, Heo, HJ, Ben-Avraham, D, Mazor, M, Budagov, T, Einstein, FH & Atzmon, G 2014, 'Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat', Molecular Genetics and Genomics, vol. 289, no. 4, pp. 695-705. https://doi.org/10.1007/s00438-014-0841-5
Bashiri, Asher ; Heo, Hye J. ; Ben-Avraham, Danny ; Mazor, Moshe ; Budagov, Temuri ; Einstein, Francine H. ; Atzmon, Gil. / Pregnancy complicated by obesity induces global transcript expression alterations in visceral and subcutaneous fat. In: Molecular Genetics and Genomics. 2014 ; Vol. 289, No. 4. pp. 695-705.
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