Preferential Lentiviral Targeting of Astrocytes in the Central Nervous System

Michael Fassler, Itai Weissberg, Nitzan Levy, Felipe Diaz-Griffero, Alon Monsonego, Alon Friedman, Ran Taube

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ability to visualize and genetically manipulate specific cell populations of the central nervous system (CNS) is fundamental to a better understanding of brain functions at the cellular and molecular levels. Tools to selectively target cells of the CNS include molecular genetics, imaging, and use of transgenic animals. However, these approaches are technically challenging, time consuming, and difficult to control. Viral-mediated targeting of cells in the CNS can be highly beneficial for studying and treating neurodegenerative diseases. Yet, despite specific marking of numerous cell types in the CNS, in vivo selective targeting of astrocytes has not been optimized. In this study, preferential targeting of astrocytes in the CNS was demonstrated using engineered lentiviruses that were pseudotyped with a modified Sindbis envelope and displayed anti-GLAST IgG on their surfaces as an attachment moiety. Viral tropism for astrocytes was initially verified in vitro in primary mixed glia cultures. When injected into the brains of mice, lentiviruses that displayed GLAST IgG on their surface, exhibited preferential astrocyte targeting, compared to pseudotyped lentiviruses that did not incorporate any IgG or that expressed a control isotype IgG. Overall, this approach is highly flexible and can be exploited to selectively target astrocytes or other cell types of the CNS. As such, it can open a window to visualize and genetically manipulate astrocytes or other cells of the CNS as means of research and treatment.

Original languageEnglish (US)
Article numbere76092
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 2 2013

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astrocytes
Neurology
Astrocytes
central nervous system
Central Nervous System
Lentivirus
Immunoglobulin G
cells
Brain
Viral Tropism
Neurodegenerative diseases
brain
tropisms
transgenic animals
Genetically Modified Animals
Molecular Imaging
mixed culture
neurodegenerative diseases
Neuroglia
molecular genetics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fassler, M., Weissberg, I., Levy, N., Diaz-Griffero, F., Monsonego, A., Friedman, A., & Taube, R. (2013). Preferential Lentiviral Targeting of Astrocytes in the Central Nervous System. PLoS One, 8(10), [e76092]. https://doi.org/10.1371/journal.pone.0076092

Preferential Lentiviral Targeting of Astrocytes in the Central Nervous System. / Fassler, Michael; Weissberg, Itai; Levy, Nitzan; Diaz-Griffero, Felipe; Monsonego, Alon; Friedman, Alon; Taube, Ran.

In: PLoS One, Vol. 8, No. 10, e76092, 02.10.2013.

Research output: Contribution to journalArticle

Fassler, M, Weissberg, I, Levy, N, Diaz-Griffero, F, Monsonego, A, Friedman, A & Taube, R 2013, 'Preferential Lentiviral Targeting of Astrocytes in the Central Nervous System', PLoS One, vol. 8, no. 10, e76092. https://doi.org/10.1371/journal.pone.0076092
Fassler, Michael ; Weissberg, Itai ; Levy, Nitzan ; Diaz-Griffero, Felipe ; Monsonego, Alon ; Friedman, Alon ; Taube, Ran. / Preferential Lentiviral Targeting of Astrocytes in the Central Nervous System. In: PLoS One. 2013 ; Vol. 8, No. 10.
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