Preferential expansion of autoreactive T lymphocytes from the memory T-cell pool by IL-7

Bibiana Bielekova, Paolo A. Muraro, Ladan Golestaneh, Jeanick Pascal, Henry F. McFarland, Roland Martin

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We have developed a new technique that allows us to quantify antigen-specific T cells, and to determine their functional phenotype and origin from naive versus memory populations. Using this methodology, we have characterized a total of 286 T-cell lines specific for myelin basic protein (MBP) and influenza hemagglutinin from 16 multiple sclerosis (MS) patients and nine healthy donors. Our data support the notion that MBP-specific T cells undergo in vivo activation in MS patients and indicate a presence of immune dysregulation that renders MS patients prone to develop autoimmunity. Our methodology offers a way to study antigen-specific T-cell characteristics as a surrogate marker in immunotherapy trials. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)115-123
Number of pages9
JournalJournal of Neuroimmunology
Volume100
Issue number1-2
DOIs
StatePublished - Dec 1999
Externally publishedYes

Fingerprint

Interleukin-7
Multiple Sclerosis
T-Lymphocytes
Myelin Basic Protein
Antigens
Hemagglutinins
Autoimmunity
Immunotherapy
Human Influenza
Biomarkers
Tissue Donors
Phenotype
Cell Line
Population

Keywords

  • Antigen-specific T cells
  • Interleukin-7
  • Multiple sclerosis
  • Primary proliferation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Preferential expansion of autoreactive T lymphocytes from the memory T-cell pool by IL-7. / Bielekova, Bibiana; Muraro, Paolo A.; Golestaneh, Ladan; Pascal, Jeanick; McFarland, Henry F.; Martin, Roland.

In: Journal of Neuroimmunology, Vol. 100, No. 1-2, 12.1999, p. 115-123.

Research output: Contribution to journalArticle

Bielekova, Bibiana ; Muraro, Paolo A. ; Golestaneh, Ladan ; Pascal, Jeanick ; McFarland, Henry F. ; Martin, Roland. / Preferential expansion of autoreactive T lymphocytes from the memory T-cell pool by IL-7. In: Journal of Neuroimmunology. 1999 ; Vol. 100, No. 1-2. pp. 115-123.
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