Predicting disulfide bond connectivity in proteins by correlated mutations analysis

Rotem Rubinstein, Andras Fiser

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Motivation: Prediction of disulfide bond connectivity facilitates structural and functional annotation of proteins. Previous studies suggest that cysteines of a disulfide bond mutate in a correlated manner. Results: We developed a method that analyzes correlated mutation patterns in multiple sequence alignments in order to predict disulfide bond connectivity. Proteins with known experimental structures and varying numbers of disulfide bonds, and that spanned various evolutionary distances, were aligned. We observed frequent variation of disulfide bond connectivity within members of the same protein families, and it was also observed that in 99% of the cases, cysteine pairs forming non-conserved disulfide bonds mutated in concert. Our data support the notion that substitution of a cysteine in a disulfide bond prompts the substitution of its cysteine partner and that oxidized cysteines appear in pairs. The method we developed predicts disulfide bond connectivity patterns with accuracies of 73, 69 and 61% for proteins with two, three and four disulfide bonds, respectively.

Original languageEnglish (US)
Pages (from-to)498-504
Number of pages7
JournalBioinformatics
Volume24
Issue number4
DOIs
StatePublished - Feb 2008

Fingerprint

Disulfides
Connectivity
Mutation
Proteins
Protein
Cysteine
Substitution
Substitution reactions
Predict
Multiple Sequence Alignment
Annotation
Molecular Sequence Annotation
Prediction
Sequence Alignment

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Predicting disulfide bond connectivity in proteins by correlated mutations analysis. / Rubinstein, Rotem; Fiser, Andras.

In: Bioinformatics, Vol. 24, No. 4, 02.2008, p. 498-504.

Research output: Contribution to journalArticle

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