Preclinical evaluation of the BET bromodomain inhibitor BAY 1238097 for the treatment of lymphoma

Elena Bernasconi, Eugenio Gaudio, Pascale Lejeune, Chiara Tarantelli, Luciano Cascione, Ivo Kwee, Filippo Spriano, Andrea Rinaldi, Afua A. Mensah, Elaine Y. L. Chung, Anastasios Stathis, Stephan Siegel, Norbert Schmees, Matthias Ocker, Emanuele Zucca, Bernard Haendler, Francesco Bertoni

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

The epigenome is often deregulated in cancer and treatment with inhibitors of bromodomain and extra-terminal proteins, the readers of epigenetic acetylation marks, represents a novel therapeutic approach. Here, we have characterized the anti-tumour activity of the novel bromodomain and extra-terminal (BET) inhibitor BAY 1238097 in preclinical lymphoma models. BAY 1238097 showed anti-proliferative activity in a large panel of lymphoma-derived cell lines, with a median 50% inhibitory concentration between 70 and 208 nmol/l. The compound showed strong anti-tumour efficacy in vivo as a single agent in two diffuse large B cell lymphoma models. Gene expression profiling showed BAY 1238097 targeted the NFKB/TLR/JAK/STAT signalling pathways, MYC and E2F1-regulated genes, cell cycle regulation and chromatin structure. The gene expression profiling signatures also highly overlapped with the signatures obtained with other BET Bromodomain inhibitors and partially overlapped with HDAC-inhibitors, mTOR inhibitors and demethylating agents. Notably, BAY 1238097 presented in vitro synergism with EZH2, mTOR and BTK inhibitors. In conclusion, the BET inhibitor BAY 1238097 presented promising anti-lymphoma preclinical activity in vitro and in vivo, mediated by the interference with biological processes driving the lymphoma cells. Our data also indicate the use of combination schemes targeting EZH2, mTOR and BTK alongside BET bromodomains.

Original languageEnglish (US)
Pages (from-to)936-948
Number of pages13
JournalBritish Journal of Haematology
Volume178
Issue number6
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

Keywords

  • BET Bromodomain
  • Epigenetics
  • EZH2
  • ibrutinib
  • Lymphomas
  • mTOR

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Bernasconi, E., Gaudio, E., Lejeune, P., Tarantelli, C., Cascione, L., Kwee, I., Spriano, F., Rinaldi, A., Mensah, A. A., Chung, E. Y. L., Stathis, A., Siegel, S., Schmees, N., Ocker, M., Zucca, E., Haendler, B., & Bertoni, F. (2017). Preclinical evaluation of the BET bromodomain inhibitor BAY 1238097 for the treatment of lymphoma. British Journal of Haematology, 178(6), 936-948. https://doi.org/10.1111/bjh.14803