PRC1 preserves epidermal tissue integrity independently of PRC2

Idan Cohen; Dejian Zhao; Gopinathan Menon; Manabu Nakayama; Haruhiko Koseki; Deyou Zheng; Elena Ezhkova

doi:10.1101/gad.319939.118

PRC1 preserves epidermal tissue integrity independently of PRC2

Idan Cohen, Dejian Zhao, Gopinathan Menon, Manabu Nakayama, Haruhiko Koseki, Deyou Zheng, Elena Ezhkova

Neurology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Polycomb-repressive complex 1 (PRC1) and PRC2 are critical chromatin regulators of gene expression and tissue development. Here, we show that despite extensive genomic cobinding, PRC1 is essential for epidermal integrity, whereas PRC2 is dispensable. Loss of PRC1 resulted in blistering skin, reminiscent of human skin fragility syndromes. Conversely, PRC1 does not restrict epidermal stratification during skin morphogenesis, whereas PRC2 does. Molecular dissection demonstrated that PRC1 functions with PRC2 to silence/dampen expression of adhesion genes. In contrast, PRC1 promotes expression of critical epidermal adhesion genes independently of PRC2-mediated H3K27me3. Together, we demonstrate a functional link between epigenetic regulation and skin diseases.

Original language	English (US)
Pages (from-to)	55-60
Number of pages	6
Journal	Genes and Development
Volume	33
Issue number	1-2
DOIs	https://doi.org/10.1101/gad.319939.118
State	Published - Jan 1 2019

Keywords

Cell adhesion
Epidermis
Epigenetics
PRC1
PRC2
Polycomb complex
Skin

ASJC Scopus subject areas

Genetics
Developmental Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1101/gad.319939.118

Cite this

@article{3f256fa4627b499e8bdba1b6313eacc6,

title = "PRC1 preserves epidermal tissue integrity independently of PRC2",

abstract = "Polycomb-repressive complex 1 (PRC1) and PRC2 are critical chromatin regulators of gene expression and tissue development. Here, we show that despite extensive genomic cobinding, PRC1 is essential for epidermal integrity, whereas PRC2 is dispensable. Loss of PRC1 resulted in blistering skin, reminiscent of human skin fragility syndromes. Conversely, PRC1 does not restrict epidermal stratification during skin morphogenesis, whereas PRC2 does. Molecular dissection demonstrated that PRC1 functions with PRC2 to silence/dampen expression of adhesion genes. In contrast, PRC1 promotes expression of critical epidermal adhesion genes independently of PRC2-mediated H3K27me3. Together, we demonstrate a functional link between epigenetic regulation and skin diseases.",

keywords = "Cell adhesion, Epidermis, Epigenetics, PRC1, PRC2, Polycomb complex, Skin",

author = "Idan Cohen and Dejian Zhao and Gopinathan Menon and Manabu Nakayama and Haruhiko Koseki and Deyou Zheng and Elena Ezhkova",

note = "Funding Information: We thank Ya-Chieh Hsu, Jose Silva, Sergei Ezhkov, and Venu Pothula for help and critical suggestions. We are grateful for the assistance and reagents provided by Julie Segre and Elaine Fuchs. We thank Miguel Vidal for the Ring1a−/− and Ring1bflox/flox mice, and Weipeng Mu and Terry Magnuson for the Eedflox/flox mice. We thank the Flow Cytometry Core Facility as well as Ronald Gordon from the Electron Microscopy Facility at the Icahn School of Medicine at Mount Sinai. Research reported here was supported by the National Institutes of Health (NIH)/National Institute of Arthritis and Musculoskeletal and Skin Diseases under award number R01 AR069078 (to E.E.), NIH/National Heart, Lung, and Blood Institute grant R01HL133120 (to D. Zheng), and the Tisch Cancer Institute P30 Cancer Support Grant (to E.E.). Publisher Copyright: {\textcopyright} 2019 Cohen et al.",

year = "2019",

month = jan,

day = "1",

doi = "10.1101/gad.319939.118",

language = "English (US)",

volume = "33",

pages = "55--60",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

number = "1-2",

}

TY - JOUR

T1 - PRC1 preserves epidermal tissue integrity independently of PRC2

AU - Cohen, Idan

AU - Zhao, Dejian

AU - Menon, Gopinathan

AU - Nakayama, Manabu

AU - Koseki, Haruhiko

AU - Zheng, Deyou

AU - Ezhkova, Elena

N1 - Funding Information: We thank Ya-Chieh Hsu, Jose Silva, Sergei Ezhkov, and Venu Pothula for help and critical suggestions. We are grateful for the assistance and reagents provided by Julie Segre and Elaine Fuchs. We thank Miguel Vidal for the Ring1a−/− and Ring1bflox/flox mice, and Weipeng Mu and Terry Magnuson for the Eedflox/flox mice. We thank the Flow Cytometry Core Facility as well as Ronald Gordon from the Electron Microscopy Facility at the Icahn School of Medicine at Mount Sinai. Research reported here was supported by the National Institutes of Health (NIH)/National Institute of Arthritis and Musculoskeletal and Skin Diseases under award number R01 AR069078 (to E.E.), NIH/National Heart, Lung, and Blood Institute grant R01HL133120 (to D. Zheng), and the Tisch Cancer Institute P30 Cancer Support Grant (to E.E.). Publisher Copyright: © 2019 Cohen et al.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Polycomb-repressive complex 1 (PRC1) and PRC2 are critical chromatin regulators of gene expression and tissue development. Here, we show that despite extensive genomic cobinding, PRC1 is essential for epidermal integrity, whereas PRC2 is dispensable. Loss of PRC1 resulted in blistering skin, reminiscent of human skin fragility syndromes. Conversely, PRC1 does not restrict epidermal stratification during skin morphogenesis, whereas PRC2 does. Molecular dissection demonstrated that PRC1 functions with PRC2 to silence/dampen expression of adhesion genes. In contrast, PRC1 promotes expression of critical epidermal adhesion genes independently of PRC2-mediated H3K27me3. Together, we demonstrate a functional link between epigenetic regulation and skin diseases.

AB - Polycomb-repressive complex 1 (PRC1) and PRC2 are critical chromatin regulators of gene expression and tissue development. Here, we show that despite extensive genomic cobinding, PRC1 is essential for epidermal integrity, whereas PRC2 is dispensable. Loss of PRC1 resulted in blistering skin, reminiscent of human skin fragility syndromes. Conversely, PRC1 does not restrict epidermal stratification during skin morphogenesis, whereas PRC2 does. Molecular dissection demonstrated that PRC1 functions with PRC2 to silence/dampen expression of adhesion genes. In contrast, PRC1 promotes expression of critical epidermal adhesion genes independently of PRC2-mediated H3K27me3. Together, we demonstrate a functional link between epigenetic regulation and skin diseases.

KW - Cell adhesion

KW - Epidermis

KW - Epigenetics

KW - PRC1

KW - PRC2

KW - Polycomb complex

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=85059499433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059499433&partnerID=8YFLogxK

U2 - 10.1101/gad.319939.118

DO - 10.1101/gad.319939.118

M3 - Article

C2 - 30567998

AN - SCOPUS:85059499433

SN - 0890-9369

VL - 33

SP - 55

EP - 60

JO - Genes and Development

JF - Genes and Development

IS - 1-2

ER -

PRC1 preserves epidermal tissue integrity independently of PRC2

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this