pp60(c-src) has less affinity for the detergent-insoluble cellular matrix than do pp60(v-src) and other viral protein-tyrosine kinases

David M. Loeb, J. Woolford, K. Beemon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A difference in affinity for a Nonidet P-40-insoluble cellular matrix was observed between the products of the viral and cellular src genes. It has previously been demonstrated that pp60(v-src) is associated with a detergent-insoluble matrix containing the cellular cytoskeleton. (J.G. Burr, G. Dreyfuss, S. Penman, and J.M. Buchanan, Proc. Natl. Acad. Sci. USA 77:3488, 1980). We observed a similar association of the transforming proteins of Fujinami sarcoma virus (P130(gag-fps)) and Yamaguchi 73 avian sarcoma virus (P90(gag-yes)), both of which are tyrosine-specific protein kinases. However, we found that the endogenous c-src product, pp60(c-src), was not tightly bound to the detergent-insoluble matrix. This does not appear to have been due to differences in the cytoskeleton between transformed and nontransformed cells since pp60(c-src) was also solubilized by nonionic detergent in cells transformed by Rous sarcoma virus. This difference in the affinities of the v-src and c-src products for cytoskeletal proteins may contribute to the inability of pp60(c-src) to transform cells.

Original languageEnglish (US)
Pages (from-to)2420-2427
Number of pages8
JournalJournal of Virology
Volume61
Issue number8
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Oncogene Protein pp60(v-src)
Proto-Oncogene Proteins pp60(c-src)
protein-tyrosine kinases
viral proteins
Viral Proteins
Avian Sarcoma Viruses
detergents
Detergents
Protein-Tyrosine Kinases
Rous sarcoma virus
cytoskeleton
Cytoskeleton
src Genes
cytoskeletal proteins
Cytoskeletal Proteins
cells
protein kinases
tyrosine
Proteins
genes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

pp60(c-src) has less affinity for the detergent-insoluble cellular matrix than do pp60(v-src) and other viral protein-tyrosine kinases. / Loeb, David M.; Woolford, J.; Beemon, K.

In: Journal of Virology, Vol. 61, No. 8, 01.01.1987, p. 2420-2427.

Research output: Contribution to journalArticle

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