Potentiation of radiation effects on multicellular tumor spheroids (mts) of hela cells by lonidamine

Jae Ho Kim, Sang Hie Kim, S. Q. He, Alan A. Alfieri, Charles W. Young

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lonidamine is a potent inhibitor of spermatogenesis and a hyperthermic sensitizer. The previous study of lonidamine and radiation using two murine tumors demonstrated that tumor cure rates were significantly increased by radiation and concomitant lonidamine. In an effort to determine the radiobiologic factors involved with the potentiating effect of radiation by lonidamine, a series of cell culture studies were carried out using multicellular tumor spheroids (MTS) of HeLa Cells. When the MTS were treated with lonidamine in combination with fractionated irradiation, remarkable enhancement of growth inhibition was observed at the drug concentration of 10 μ/ml. On the other hand, there was no demonstrable enhancement of growth inhibition induced by a single dose of irradiation. Although the present findings would be consistent with the inhibitory action of potentially lethal damage repair of radiation by the drug, an alternative possibility is that the cells that have received the combined treatment have undergone a metabolic change, which has altered their sensitivity to the growth inhibitory effects of lonidamine. Based on the studies reported here and in mice, it is suggested that continued drug exposure over a prolonged period may provide an enhanced therapeutic effect, even in tumor varieties where the drug has no apparent antitumor activity on nonirradiated cells.

Original languageEnglish (US)
Pages (from-to)1277-1280
Number of pages4
JournalInternational Journal of Radiation Oncology, Biology, Physics
Volume16
Issue number5
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Cellular Spheroids
spheroids
Radiation Effects
radiation effects
HeLa Cells
tumors
drugs
cells
radiation
Neoplasms
Radiation
Pharmaceutical Preparations
spermatogenesis
Growth
irradiation
augmentation
inhibitors
Therapeutic Uses
mice
Spermatogenesis

Keywords

  • Fractionated radiation
  • Lonidamine
  • Tumor spheroids

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Potentiation of radiation effects on multicellular tumor spheroids (mts) of hela cells by lonidamine. / Kim, Jae Ho; Kim, Sang Hie; He, S. Q.; Alfieri, Alan A.; Young, Charles W.

In: International Journal of Radiation Oncology, Biology, Physics, Vol. 16, No. 5, 1989, p. 1277-1280.

Research output: Contribution to journalArticle

Kim, Jae Ho ; Kim, Sang Hie ; He, S. Q. ; Alfieri, Alan A. ; Young, Charles W. / Potentiation of radiation effects on multicellular tumor spheroids (mts) of hela cells by lonidamine. In: International Journal of Radiation Oncology, Biology, Physics. 1989 ; Vol. 16, No. 5. pp. 1277-1280.
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