Potassium and taurine release are highly correlated with regulatory volume decrease in neonatal primary rat astrocyte cultures

Domenico Vitarella, Darryl J. DiRisio, Harold K. Kimelberg, Michael Aschner

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

Neonatal rat primary astrocyte cultures were swollen by exposure to hypotonic buffer. Using an electrical impedance method for determination of cell volume coupled with on-line measurements of efflux of radioactive ions or amino acids, we have investigated the role of K+ (using 86Rb), taurine, and D-aspartate (an analogue of glutamate) in regulatory volume decrease (RVD). Addition of 1 mM quinine, 10 μM nimodipine, 100 μM BAPTA-AM, 10 μM trifluoperazine, or a calcium-free buffer significantly (p < 0.0001) inhibited RVD. This was accompanied by inhibition of 86Rb release but an increase in D-[3H]aspartate release, which was proportional to the degree to which RVD was inhibited. These results support a regulatory role for calcium in RVD and show that inhibition of calcium entry from the extracellular fluid, intracellular calcium sequestration, inhibition of calcium-activated K+ channels, and inhibition of calmodulin all inhibit RVD. Because D- [3H]aspartate efflux profiles increase as RVD is inhibited, it is unlikely that D-aspartate release is a main determinant of RVD. In contrast, [3H]taurine release was increased by 1 mM quinine and inhibited by 10 μM trifluoperazine. The net release of K+ and taurine is highly correlated with the degree of RVD, implicating a regulatory role for both K+ and taurine release in RVD.

Original languageEnglish (US)
Pages (from-to)1143-1149
Number of pages7
JournalJournal of Neurochemistry
Volume63
Issue number3
DOIs
StatePublished - Sep 1994
Externally publishedYes

Keywords

  • Aspartate
  • Astrocytes
  • Calcium
  • Taurine
  • Volume regulation

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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