Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus

D. Frank, E. Cesarman, Fang Liu Yi Fang Liu, Robert E. Michler, D. M. Knowles

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Two families of Epstein-Barr virus (EBV), type A and type B, have been defined on the basis of sequence divergence in the EBNA-2 gene. Type A EBV immortalizes B cells more efficiently in vitro and infects immunocompetent individuals more commonly than type B EBV. However, increased rates of infection by type B EBV are seen in immunocompromised hosts and in many lymphoid neoplasms associated with immunocompromise. The posttransplantation lymphoproliferative disorders (PT-LPDs) are a heterogeneous group of B-cell neoplasms that arise in the setting of immunosuppressive therapy, and are associated with EBV infection. Whether type A and/or type B EBV are associated with PT-LPDs is unknown. Therefore, we investigated 27 PT-LPD lesions from 22 solid-organ transplant recipients by polymerase chain reaction (PCR) at the EBNA-2 and EBNA-3c loci to detect sequence deletions that distinguish the two EBV families. Another locus, EBER, was examined by single-strand conformation polymorphism analysis (SSCP), in conjunction with direct sequencing in selected cases. Type A EBV was found in 24 of 27 cases (89%) as seen by amplification of the EBNA-2 and EBNA-3c regions. Four different EBER polymorphisms were detected, confirming the presence of different type A EBV isolates among these cases. Three cases were negative for infection by EBV. Surprisingly, despite the immunocompromised state of the hosts, none of the 27 PT-LPD lesions harbored type B EBV. Thus, although type B EBV may commonly infect peripheral blood lymphocytes in immunocompromised individuals, they do not appear to induce readily PT-LPD formation.

Original languageEnglish (US)
Pages (from-to)1396-1403
Number of pages8
JournalBlood
Volume85
Issue number5
StatePublished - 1995
Externally publishedYes

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Cercopithecine Herpesvirus 1
Lymphoproliferative Disorders
Human Herpesvirus 4
Viruses
Epstein-Barr Virus Infections
Immunocompromised Host
B-Lymphocytes
Polymorphism
Sequence Deletion
Cells
Immunosuppressive Agents
Transplants
Lymphocytes
Polymerase chain reaction
Neoplasms
Amplification
Conformations
Polymerase Chain Reaction
Blood

ASJC Scopus subject areas

  • Hematology

Cite this

Frank, D., Cesarman, E., Yi Fang Liu, F. L., Michler, R. E., & Knowles, D. M. (1995). Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus. Blood, 85(5), 1396-1403.

Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus. / Frank, D.; Cesarman, E.; Yi Fang Liu, Fang Liu; Michler, Robert E.; Knowles, D. M.

In: Blood, Vol. 85, No. 5, 1995, p. 1396-1403.

Research output: Contribution to journalArticle

Frank, D, Cesarman, E, Yi Fang Liu, FL, Michler, RE & Knowles, DM 1995, 'Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus', Blood, vol. 85, no. 5, pp. 1396-1403.
Frank, D. ; Cesarman, E. ; Yi Fang Liu, Fang Liu ; Michler, Robert E. ; Knowles, D. M. / Posttransplantation lymphoproliferative disorders frequently contain type A and not type B Epstein-Barr virus. In: Blood. 1995 ; Vol. 85, No. 5. pp. 1396-1403.
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