Postprandial oxidative stress is modified by dietary fat: Evidence from a human intervention study

Pablo Perez-Martinez, Jose Maria Garcia-Quintana, Elena M. Yubero-Serrano, Inmaculada Tasset-Cuevas, Isaac Tunez, Antonio Garcia-Rios, Javier Delgado-Lista, Carmen Marin, Francisco Perez-Jimenez, Helen M. Roche, Jose Lopez-Miranda

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


Previous evidence supports the concept that increased oxidative stress may play an important role in MetS (metabolic syndrome)-related manifestations. Dietary fat quality has been proposed to be critical in oxidative stress and the pathogenesis of the MetS. In the present study, we investigated whether oxidative stress parameters are affected by diets with different fat quantity and quality during the postprandial state in subjects with the MetS. Patients were randomly assigned to one of four isoenergetic diets distinct in fat quantity and quality for 12 weeks: a high-saturated-fatty-acid (HSFA) diet, a high-mono-unsaturated-fatty-acid (HMUFA) diet and two low-fat/high-complex carbohydrate diets [supplemented with 1.24 g/day of long-chain n-3 polyunsaturated fatty acid (LFHCC n-3) or with 1 g/day of sunflower oil high in oleic acid (LFHCC) as placebo]. The HMUFA diet enhanced postprandial GSH (reduced glutathione) levels and the GSH/GSSH (oxidized glutathione) ratio, compared with the other three diets. In addition, after the HMUFA-rich diet postprandial lipid peroxide levels, protein carbonyl concentrations, SOD (superoxide dismutase) activity and plasma H2O2 levels were lower compared with subjects adhering to the HSFA-rich diet. Both LFHCC diets had an intermediate effect relative to the HMUFA and HSFA diets. In conclusion, our data support the notion that the HMUFA diet improves postprandial oxidative stress in patients with the MetS. These findings suggest that the postprandial state is important for understanding the possible cardioprotective effects associated with mono-unsaturated dietary fat, particularly in subjects with the MetS.

Original languageEnglish (US)
Pages (from-to)251-261
Number of pages11
JournalClinical Science
Issue number6
StatePublished - Sep 2010
Externally publishedYes


  • Dietary fat
  • LIPGENE study
  • Metabolic syndrome
  • Oxidative stress
  • Postprandial lipaemia

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Postprandial oxidative stress is modified by dietary fat: Evidence from a human intervention study'. Together they form a unique fingerprint.

Cite this