Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice

Jeffrey W. Ryder, Yuichi Kawano, Dana Galuska, Roger Fahlman, Harriet Wallberg-Henriksson, Maureen J. Charron, Juleen R. Zierath

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

To determine the role of GLUT4 on postexercise glucose transport and glycogen resynthesis in skeletal muscle, GLUT4-deficient and wild-type mice were studied after a 3 h swim exercise. In wild-type mice, insulin and swimming each increased 2-deoxyglucose uptake by twofold in extensor digitorum longus muscle. In contrast, insulin did not increase 2-deoxyglucose glucose uptake in muscle from GLUT4-null mice. Swimming increased glucose transport twofold in muscle from fed GLUT4-null mice, with no effect noted in fasted GLUT4-null mice. This exercise-associated 2-deoxyglucose glucose uptake was not accompanied by increased cell surface GLUT1 content. Glucose transport in GLUT4-null muscle was increased 1.6-fold over basal levels after electrical stimulation. Contraction-induced glucose transport activity was fourfold greater in wild-type vs. GLUT4-null muscle. Glycogen content in gastrocnemius muscle was similar between wild-type and GLUT4-null mice and was reduced ~50% after exercise. After 5 h carbohydrate refeeding, muscle glycogen content was fully restored in wild-type, with no change in GLUT4- null mice. After 24 h carbohydrate refeeding, muscle glycogen in GLUT4-null mice was restored to fed levels. In conclusion, GLUT4 is the major transporter responsible for exercise-induced glucose transport. Also, postexercise glycogen resynthesis in muscle was greatly delayed; unlike wild- type mice, glycogen supercompensation was not found. GLUT4 it is not essential for glycogen repletion since muscle glycogen levels in previously exercised GLUT4-null mice were totally restored after 24 h carbohydrate refeeding. - Ryder, J. W., Kawano, Y., Galuska, D., Fahlman, R., Wallberg- Henriksson, H., Charron, M. J., Zierath, J. R. Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice.

Original languageEnglish (US)
Pages (from-to)2246-2256
Number of pages11
JournalFASEB Journal
Volume13
Issue number15
StatePublished - 1999

Fingerprint

Glycogen
glycogen
Muscle
skeletal muscle
Skeletal Muscle
uptake mechanisms
Glucose
glucose
synthesis
muscles
mice
Muscles
2-deoxyglucose
refeeding
Deoxyglucose
exercise
Carbohydrates
carbohydrates
insulin
Insulin

Keywords

  • Electrical stimulation
  • Glucose transport
  • Glycogen synthase
  • Metabolism
  • Physical exercise

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Ryder, J. W., Kawano, Y., Galuska, D., Fahlman, R., Wallberg-Henriksson, H., Charron, M. J., & Zierath, J. R. (1999). Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice. FASEB Journal, 13(15), 2246-2256.

Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice. / Ryder, Jeffrey W.; Kawano, Yuichi; Galuska, Dana; Fahlman, Roger; Wallberg-Henriksson, Harriet; Charron, Maureen J.; Zierath, Juleen R.

In: FASEB Journal, Vol. 13, No. 15, 1999, p. 2246-2256.

Research output: Contribution to journalArticle

Ryder, JW, Kawano, Y, Galuska, D, Fahlman, R, Wallberg-Henriksson, H, Charron, MJ & Zierath, JR 1999, 'Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice', FASEB Journal, vol. 13, no. 15, pp. 2246-2256.
Ryder JW, Kawano Y, Galuska D, Fahlman R, Wallberg-Henriksson H, Charron MJ et al. Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice. FASEB Journal. 1999;13(15):2246-2256.
Ryder, Jeffrey W. ; Kawano, Yuichi ; Galuska, Dana ; Fahlman, Roger ; Wallberg-Henriksson, Harriet ; Charron, Maureen J. ; Zierath, Juleen R. / Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice. In: FASEB Journal. 1999 ; Vol. 13, No. 15. pp. 2246-2256.
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