Postembedding immunolabeling of thin sections of Drosophila tissues for transmission electron microscopy

Kent L. McDonald, David J. Sharp, Wayne Rickoll

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Postembedding immunolabeling using resin sections is the recommended method for beginners carrying out electron microscopy (EM) immunolabeling. Postembedding labeling refers to labeling on sections, which is a method of gaining access to the interior of the cell without the harshness of detergent or ionic extraction as is performed with preembed labeling. Investigators already familiar with routine EM-sectioning techniques find EM immunolabeling using resin sections easiest to do, as procedures are similar to those used when performing light microscopy (LM) immunolabeling, but using a different resin. In addition, the overall preservation of structure is best in resin compared to use of cryosections or preembed labeling. The most critical component of immunoEM (iEM) is what primary antibody to use. This protocol descibes antibody labeling procedures for postembedding iEM using thin sections of Drosophila tissues.

Original languageEnglish (US)
Pages (from-to)810-812
Number of pages3
JournalCold Spring Harbor Protocols
Volume7
Issue number7
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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