Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies

Christopher Kim, Bryan A. Bassig, Wei Jie Seow, Wei Hu, Mark P. Purdue, Xiao Ou Shu, Wen Yi Huang, Chin San Liu, Wen Ling Cheng, Ta Tsung Lin, Yong Bing Xiang, Bu Tian Ji, Yu Tang Gao, Wong Ho Chow, Satu Männistö, Stephanie J. Weinstein, Demetrius Albanes, Wei Zheng, Howard D. Hosgood, Unhee Lim & 2 others Nathaniel Rothman, Qing Lan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Results: Overall, mtDNA CN was not associated with lung cancer risk in the PLCO, SWHS, or pooled populations (all P trends > 0.42, P heterogeneity = 0.0001), and mtDNA CN was inversely associated with lung cancer risk among male smokers in PLCO, the opposite direction observed in ATBC. In addition, the mtDNA CN association observed among male heavy smokers in ATBC was the opposite direction in PLCO.

Conclusions: mtDNA CN was not consistently associated with lung cancer risk across three prospective study populations from Europe, Asia, and the United States.

Impact: This pooled study suggests no consistent association between prediagnostic mtDNA CN levels and lung cancer risk across several populations.

Background: We previously reported that higher levels of mitochondrial DNA copy number (mtDNA CN) were associated with lung cancer risk among male heavy smokers (i.e., ≥20 cigarettes per day) in the Alpha-Tocopherol Beta-Carotene (ATBC) study. Here, we present two additional prospective investigations nested in the Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial and the Shanghai Women's Health Study (SWHS), and pooled with previously published data from ATBC.

Materials: All DNA were extracted from peripheral whole blood samples using the phenol-chloroform method, and mtDNA CN was assayed by fluorescence-based qPCR. Multivariate unconditional logistic regression models were used to estimate ORs and 95% confidence intervals for the association of mtDNA CN and lung cancer risk.

Original languageEnglish (US)
Pages (from-to)2977-2980
Number of pages4
JournalCancer Epidemiology Biomarkers and Prevention
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2014

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Mitochondrial DNA
Lung Neoplasms
Prospective Studies
beta Carotene
alpha-Tocopherol
Prostate
Women's Health
Lung
Logistic Models
Population
Chloroform
Phenol
Early Detection of Cancer
Tobacco Products
Ovarian Neoplasms
Colorectal Neoplasms
Prostatic Neoplasms
Fluorescence
Confidence Intervals
DNA

ASJC Scopus subject areas

  • Epidemiology
  • Oncology

Cite this

Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies. / Kim, Christopher; Bassig, Bryan A.; Seow, Wei Jie; Hu, Wei; Purdue, Mark P.; Shu, Xiao Ou; Huang, Wen Yi; Liu, Chin San; Cheng, Wen Ling; Lin, Ta Tsung; Xiang, Yong Bing; Ji, Bu Tian; Gao, Yu Tang; Chow, Wong Ho; Männistö, Satu; Weinstein, Stephanie J.; Albanes, Demetrius; Zheng, Wei; Hosgood, Howard D.; Lim, Unhee; Rothman, Nathaniel; Lan, Qing.

In: Cancer Epidemiology Biomarkers and Prevention, Vol. 23, No. 12, 01.12.2014, p. 2977-2980.

Research output: Contribution to journalArticle

Kim, C, Bassig, BA, Seow, WJ, Hu, W, Purdue, MP, Shu, XO, Huang, WY, Liu, CS, Cheng, WL, Lin, TT, Xiang, YB, Ji, BT, Gao, YT, Chow, WH, Männistö, S, Weinstein, SJ, Albanes, D, Zheng, W, Hosgood, HD, Lim, U, Rothman, N & Lan, Q 2014, 'Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies', Cancer Epidemiology Biomarkers and Prevention, vol. 23, no. 12, pp. 2977-2980. https://doi.org/10.1158/1055-9965.EPI-14-1070
Kim, Christopher ; Bassig, Bryan A. ; Seow, Wei Jie ; Hu, Wei ; Purdue, Mark P. ; Shu, Xiao Ou ; Huang, Wen Yi ; Liu, Chin San ; Cheng, Wen Ling ; Lin, Ta Tsung ; Xiang, Yong Bing ; Ji, Bu Tian ; Gao, Yu Tang ; Chow, Wong Ho ; Männistö, Satu ; Weinstein, Stephanie J. ; Albanes, Demetrius ; Zheng, Wei ; Hosgood, Howard D. ; Lim, Unhee ; Rothman, Nathaniel ; Lan, Qing. / Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies. In: Cancer Epidemiology Biomarkers and Prevention. 2014 ; Vol. 23, No. 12. pp. 2977-2980.
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title = "Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies",
abstract = "Results: Overall, mtDNA CN was not associated with lung cancer risk in the PLCO, SWHS, or pooled populations (all P trends > 0.42, P heterogeneity = 0.0001), and mtDNA CN was inversely associated with lung cancer risk among male smokers in PLCO, the opposite direction observed in ATBC. In addition, the mtDNA CN association observed among male heavy smokers in ATBC was the opposite direction in PLCO.Conclusions: mtDNA CN was not consistently associated with lung cancer risk across three prospective study populations from Europe, Asia, and the United States.Impact: This pooled study suggests no consistent association between prediagnostic mtDNA CN levels and lung cancer risk across several populations.Background: We previously reported that higher levels of mitochondrial DNA copy number (mtDNA CN) were associated with lung cancer risk among male heavy smokers (i.e., ≥20 cigarettes per day) in the Alpha-Tocopherol Beta-Carotene (ATBC) study. Here, we present two additional prospective investigations nested in the Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial and the Shanghai Women's Health Study (SWHS), and pooled with previously published data from ATBC.Materials: All DNA were extracted from peripheral whole blood samples using the phenol-chloroform method, and mtDNA CN was assayed by fluorescence-based qPCR. Multivariate unconditional logistic regression models were used to estimate ORs and 95{\%} confidence intervals for the association of mtDNA CN and lung cancer risk.",
author = "Christopher Kim and Bassig, {Bryan A.} and Seow, {Wei Jie} and Wei Hu and Purdue, {Mark P.} and Shu, {Xiao Ou} and Huang, {Wen Yi} and Liu, {Chin San} and Cheng, {Wen Ling} and Lin, {Ta Tsung} and Xiang, {Yong Bing} and Ji, {Bu Tian} and Gao, {Yu Tang} and Chow, {Wong Ho} and Satu M{\"a}nnist{\"o} and Weinstein, {Stephanie J.} and Demetrius Albanes and Wei Zheng and Hosgood, {Howard D.} and Unhee Lim and Nathaniel Rothman and Qing Lan",
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T1 - Pooled analysis of mitochondrial DNA copy number and lung cancer risk in three prospective studies

AU - Kim, Christopher

AU - Bassig, Bryan A.

AU - Seow, Wei Jie

AU - Hu, Wei

AU - Purdue, Mark P.

AU - Shu, Xiao Ou

AU - Huang, Wen Yi

AU - Liu, Chin San

AU - Cheng, Wen Ling

AU - Lin, Ta Tsung

AU - Xiang, Yong Bing

AU - Ji, Bu Tian

AU - Gao, Yu Tang

AU - Chow, Wong Ho

AU - Männistö, Satu

AU - Weinstein, Stephanie J.

AU - Albanes, Demetrius

AU - Zheng, Wei

AU - Hosgood, Howard D.

AU - Lim, Unhee

AU - Rothman, Nathaniel

AU - Lan, Qing

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Results: Overall, mtDNA CN was not associated with lung cancer risk in the PLCO, SWHS, or pooled populations (all P trends > 0.42, P heterogeneity = 0.0001), and mtDNA CN was inversely associated with lung cancer risk among male smokers in PLCO, the opposite direction observed in ATBC. In addition, the mtDNA CN association observed among male heavy smokers in ATBC was the opposite direction in PLCO.Conclusions: mtDNA CN was not consistently associated with lung cancer risk across three prospective study populations from Europe, Asia, and the United States.Impact: This pooled study suggests no consistent association between prediagnostic mtDNA CN levels and lung cancer risk across several populations.Background: We previously reported that higher levels of mitochondrial DNA copy number (mtDNA CN) were associated with lung cancer risk among male heavy smokers (i.e., ≥20 cigarettes per day) in the Alpha-Tocopherol Beta-Carotene (ATBC) study. Here, we present two additional prospective investigations nested in the Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial and the Shanghai Women's Health Study (SWHS), and pooled with previously published data from ATBC.Materials: All DNA were extracted from peripheral whole blood samples using the phenol-chloroform method, and mtDNA CN was assayed by fluorescence-based qPCR. Multivariate unconditional logistic regression models were used to estimate ORs and 95% confidence intervals for the association of mtDNA CN and lung cancer risk.

AB - Results: Overall, mtDNA CN was not associated with lung cancer risk in the PLCO, SWHS, or pooled populations (all P trends > 0.42, P heterogeneity = 0.0001), and mtDNA CN was inversely associated with lung cancer risk among male smokers in PLCO, the opposite direction observed in ATBC. In addition, the mtDNA CN association observed among male heavy smokers in ATBC was the opposite direction in PLCO.Conclusions: mtDNA CN was not consistently associated with lung cancer risk across three prospective study populations from Europe, Asia, and the United States.Impact: This pooled study suggests no consistent association between prediagnostic mtDNA CN levels and lung cancer risk across several populations.Background: We previously reported that higher levels of mitochondrial DNA copy number (mtDNA CN) were associated with lung cancer risk among male heavy smokers (i.e., ≥20 cigarettes per day) in the Alpha-Tocopherol Beta-Carotene (ATBC) study. Here, we present two additional prospective investigations nested in the Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial and the Shanghai Women's Health Study (SWHS), and pooled with previously published data from ATBC.Materials: All DNA were extracted from peripheral whole blood samples using the phenol-chloroform method, and mtDNA CN was assayed by fluorescence-based qPCR. Multivariate unconditional logistic regression models were used to estimate ORs and 95% confidence intervals for the association of mtDNA CN and lung cancer risk.

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EP - 2980

JO - Cancer Epidemiology Biomarkers and Prevention

JF - Cancer Epidemiology Biomarkers and Prevention

SN - 1055-9965

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