Polyvalent Rev decoys act as artificial Rev-responsive elements

Tonia L. Symensma, Scott Baskerville, Amy Yan, Andrew D. Ellington

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Interactions between Rev and the Rev-responsive element (RRE) control the order, rate, and extent of gene expression in human immunodeficiency virus type 1. Rev decoys may therefore prove to be useful RNA therapeutics for the treatment of AIDS. To improve upon the current generation of Rev decoys that bind single Rev molecules, it would be useful to generate polyvalent Rev decoys that could bind multiple Rev molecules. J. Kjems and P. A. Sharp (J. Virol. 67:4769-4776, 1993) originally constructed functional polyvalent Rev decoys, but the structural context of these polyvalent decoys remains unclear, and it has been argued that the individual decoys were either structurally discrete (Kjems and Sharp, J. Virol. 67:4769-4776, 1993) or were part of an extended helix (R. W. Zemmel et al., Mol. Biol. 258:763- 777, 1996). To resolve the differences between these models, we have designed and synthesized concatemers of Rev-binding elements (RBEs) that fold to form multiple, discrete, high-affinity Rev-binding sites. We find that the concatenated RBEs can facilitate the cytoplasmic transport of viral mRNAs and therefore likely bind multiple Rev molecules. These artificial RREs may simultaneously sequester Rev and hinder access to the cellular transport machinery.

Original languageEnglish (US)
Pages (from-to)4341-4349
Number of pages9
JournalJournal of virology
Volume73
Issue number5
Publication statusPublished - May 3 1999

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ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Symensma, T. L., Baskerville, S., Yan, A., & Ellington, A. D. (1999). Polyvalent Rev decoys act as artificial Rev-responsive elements. Journal of virology, 73(5), 4341-4349.