Polymorphonuclear neutrophils promote rFGF-2-induced angiogenesis in Vivo

Jason P. Shaw, Neal Chuang, Herman Yee, Peter Shamamian

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Background. The role of neutrophils in angiogenesis remains largely unknown. Recent evidence has shown that polymorphonuclear neutrophils (PMNs) produce several proangiogenic cytokines, including VEGF, TNF-α, IL-1, IL-6, and IL-8. In addition, PMN-derived proteinases promote endothelial cell migration. We hypothesized that PMNs may facilitate angiogenesis and that reducing circulating PMNs might alter the host angiogenic response. Materials and methods. We utilized a corneal pocket assay to compare rFGF-2-induced vessel formation in the corneas of mice with normal levels of circulating neutrophils to those in a neutropenic state. Circulating PMNs were reduced using serial intraperitoneal injections of monoclonal antibody to Gr-1. Slow release rFGF2 pellets were implanted into the corneas of neutropenic mice and controls. Corneal neovascularization, measured as vessel length and area of vessel in-growth, was quantified using slit-lamp microscopy on day 7. Results. The average number of circulating PMNs was significantly reduced in the experimental group compared to the control group on days 1-7 (P < 0.05). No statistical differences in circulating monocytes or lymphocytes were observed from days 0 to 6. Mice in the experimental group had a vascular area of 2.58 ± 0.2 mm2 compared to 3.55 ± 0.3 mm2 in the control group (P < 0.05). Conclusions. Corneal neovascularization in response to rFGF-2 is diminished by PMN depletion. PMNs play an important role in facilitating rFGF-2-induced angiogenesis.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalJournal of Surgical Research
Issue number1
StatePublished - Jan 2003
Externally publishedYes


  • Angiogenesis
  • Cornea
  • Endothelium
  • Neutrophil
  • Polymorphonuclear cells
  • rFGF-2

ASJC Scopus subject areas

  • Surgery


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