Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation

Wendy K. Chung, Streamson C. Chua, Gwo Hwa Lee, Rudolph L. Leibe

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C→T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A→C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.

Original languageEnglish (US)
Pages (from-to)183-185
Number of pages3
JournalObesity research
Volume5
Issue number3
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

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Keywords

  • Lep
  • Leptin
  • Obesity
  • ob

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Public Health, Environmental and Occupational Health

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