Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation

Wendy K. Chung, Streamson C. Chua, Jr., Gwo Hwa Lee, Rudolph L. Leibe

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Three polymerase chain reaction-based assays for the mouse Lepob mutation are described: Dde I site created by the C→T transversion characterizing Lepob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A→C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.

Original languageEnglish (US)
Pages (from-to)183-185
Number of pages3
JournalObesity Research
Volume5
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Obese Mice
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
polymerase chain reaction
Alleles
alleles
mutation
Polymerase Chain Reaction
Mutation
mice
assays
Heterozygote
heterozygosity
mutants

Keywords

  • Lep
  • Leptin
  • ob
  • Obesity

ASJC Scopus subject areas

  • Food Science
  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Public Health, Environmental and Occupational Health

Cite this

Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lepob) mutation. / Chung, Wendy K.; Chua, Jr., Streamson C.; Lee, Gwo Hwa; Leibe, Rudolph L.

In: Obesity Research, Vol. 5, No. 3, 1997, p. 183-185.

Research output: Contribution to journalArticle

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