Polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) and electrophoretic assays for the mouse obese (Lep^ob) mutation

Three polymerase chain reaction-based assays for the mouse Lep^ob mutation are described: Dde I site created by the C→T transversion characterizing Lep^ob enables positive detection of the mutant allele; positive detection of the wild-type Lep allele is achieved by the use of primer sequence which introduces an A→C substitution, creating an Msp I site in the normal allele; and an electrophoretic assay which positively identifies the heterozygote.