Polymerase chain reaction detection of the Dde I polymorphism in the factor 9 gene for fragile X linkage analysis

C. S. Dobkin; M. C. Driscoll; C. Ferrando

doi:10.1002/ajmg.1320380244

Polymerase chain reaction detection of the Dde I polymorphism in the factor 9 gene for fragile X linkage analysis

C. S. Dobkin, M. C. Driscoll, C. Ferrando

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by agarose gel electrophoresis. PCR detection of this polymorphism is much simpler, more accurate, and quicker than conventional analysis.

Original language	English (US)
Pages (from-to)	378-379
Number of pages	2
Journal	American journal of medical genetics
Volume	38
Issue number	2-3
DOIs	https://doi.org/10.1002/ajmg.1320380244
State	Published - 1991
Externally published	Yes

Keywords

DNA polymorphism
linkage analysis
restriction enzymes

ASJC Scopus subject areas

Genetics(clinical)

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10.1002/ajmg.1320380244

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abstract = "We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by agarose gel electrophoresis. PCR detection of this polymorphism is much simpler, more accurate, and quicker than conventional analysis.",

keywords = "DNA polymorphism, linkage analysis, restriction enzymes",

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AU - Dobkin, C. S.

AU - Driscoll, M. C.

AU - Ferrando, C.

PY - 1991

Y1 - 1991

N2 - We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by agarose gel electrophoresis. PCR detection of this polymorphism is much simpler, more accurate, and quicker than conventional analysis.

AB - We have used the polymerase chain reaction (PCR) to analyze the 50 base pair (bp) insertion/deletion polymorphism in the coagulation factor 9 gene. This procedure is particularly applicable for DNA marker studies in fragile X families. The polymorphism, which can also be detected in Dde I digestions, was detected by the amplification of fragments of 298 and 348 bp. The alleles were distinguished directly by agarose gel electrophoresis. PCR detection of this polymorphism is much simpler, more accurate, and quicker than conventional analysis.

KW - DNA polymorphism

KW - linkage analysis

KW - restriction enzymes

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Polymerase chain reaction detection of the Dde I polymorphism in the factor 9 gene for fragile X linkage analysis

Abstract

Keywords

ASJC Scopus subject areas

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