Abstract
We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β-like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites - Xmnl 5' to the (G)γ gene, HindIII in the IVSII of (G)γ and A(γ) gene, HincII 3' and inside the γ gene, HinfI 5' of the β gene, and HpaI 3' of the β gene - are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost-effectiveness.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 66-69 |
| Number of pages | 4 |
| Journal | American Journal of Hematology |
| Volume | 32 |
| Issue number | 1 |
| State | Published - 1989 |
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- Hematology
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Polymerase chain reaction amplification applied to the determination of β-like globin gene cluster haplotypes. / Sutton, M.; Bouhassira, Eric E.; Nagel, R. L.
In: American Journal of Hematology, Vol. 32, No. 1, 1989, p. 66-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polymerase chain reaction amplification applied to the determination of β-like globin gene cluster haplotypes
AU - Sutton, M.
AU - Bouhassira, Eric E.
AU - Nagel, R. L.
PY - 1989
Y1 - 1989
N2 - We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β-like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites - Xmnl 5' to the (G)γ gene, HindIII in the IVSII of (G)γ and A(γ) gene, HincII 3' and inside the γ gene, HinfI 5' of the β gene, and HpaI 3' of the β gene - are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost-effectiveness.
AB - We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β-like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites - Xmnl 5' to the (G)γ gene, HindIII in the IVSII of (G)γ and A(γ) gene, HincII 3' and inside the γ gene, HinfI 5' of the β gene, and HpaI 3' of the β gene - are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost-effectiveness.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024376665&partnerID=8YFLogxK
M3 - Article
C2 - 2757004
AN - SCOPUS:0024376665
VL - 32
SP - 66
EP - 69
JO - American Journal of Hematology
JF - American Journal of Hematology
SN - 0361-8609
IS - 1
ER -