Polymerase chain reaction amplification applied to the determination of β-like globin gene cluster haplotypes

M. Sutton, Eric E. Bouhassira, R. L. Nagel

Research output: Contribution to journalArticle

270 Citations (Scopus)

Abstract

We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β-like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites - Xmnl 5' to the (G)γ gene, HindIII in the IVSII of (G)γ and A(γ) gene, HincII 3' and inside the γ gene, HinfI 5' of the β gene, and HpaI 3' of the β gene - are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost-effectiveness.

Original languageEnglish (US)
Pages (from-to)66-69
Number of pages4
JournalAmerican Journal of Hematology
Volume32
Issue number1
StatePublished - 1989

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Globins
Multigene Family
Haplotypes
Polymerase Chain Reaction
Genes
Ethidium
Agar Gel Electrophoresis
Ultraviolet Rays
Cost-Benefit Analysis
Safety
Enzymes

ASJC Scopus subject areas

  • Hematology

Cite this

Polymerase chain reaction amplification applied to the determination of β-like globin gene cluster haplotypes. / Sutton, M.; Bouhassira, Eric E.; Nagel, R. L.

In: American Journal of Hematology, Vol. 32, No. 1, 1989, p. 66-69.

Research output: Contribution to journalArticle

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