TY - JOUR
T1 - Polymerase chain reaction amplification applied to the determination of β‐like globin gene cluster haplotypes
AU - Sutton, Millicent
AU - Bouhassira, Eric E.
AU - Nagel, Ronald L.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1989/9
Y1 - 1989/9
N2 - We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β‐like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites—Xmnl 5′ to the Gγ gene, Hindlll in the IVSII of Gγ and A γ gene, Hincll 3′ and inside the γ gene, Hinfl 5′ of the β gene—and Hpal 3′ of the β gene—are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost‐effectiveness.
AB - We report here on the application of the polymerase chain reaction (PCR) technique for the determination of the β‐like gene cluster haplotypes. Seven fragments containing each one of the following polymorphic sites—Xmnl 5′ to the Gγ gene, Hindlll in the IVSII of Gγ and A γ gene, Hincll 3′ and inside the γ gene, Hinfl 5′ of the β gene—and Hpal 3′ of the β gene—are amplified using the PCR technique. Each amplified fragment is subsequently digested with the appropriate enzyme, analyzed by electrophoresis on agarose gel containing ethidium bromide, and visualized under ultraviolet light. This technique has the advantages of rapidity, safety, and cost‐effectiveness.
KW - Hb S, sickle cell anemia
UR - http://www.scopus.com/inward/record.url?scp=0024376665&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024376665&partnerID=8YFLogxK
U2 - 10.1002/ajh.2830320113
DO - 10.1002/ajh.2830320113
M3 - Article
C2 - 2757004
AN - SCOPUS:0024376665
VL - 32
SP - 66
EP - 69
JO - American Journal of Hematology
JF - American Journal of Hematology
SN - 0361-8609
IS - 1
ER -