Poleward tubulin flux in spindles: Regulation and function in mitotic cells

Daniel W. Buster, Dong Zhang, David J. Sharp

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

The poleward flux of tubulin subunits through spindle microtubules is a striking and conserved phenomenon whose function and molecular components remain poorly understood. To screen for novel components of the flux machinery, we utilized RNA interference to deplete regulators of microtubule dynamics, individually and in various combinations, from S2 cells and examined the resulting impact on flux rate. This led to the identification of two previously unknown flux inhibitors, KLP59C and KLP67A, and a flux promoter, Mini-spindles. Furthermore, we find that flux rate is regulated by functional antagonism among microtubule stabilizers and destabilizers specifically at plus ends. Finally, by examining mitosis on spindles in which flux has been up- or down-regulated or restored after the codepletion of antagonistic flux regulators, we show that flux is an integral contributor to anaphase A but is not responsible for chromosome congression, interkinetochore tension, or the establishment of normal spindle length during prometaphase/metaphase.

Original languageEnglish (US)
Pages (from-to)3094-3104
Number of pages11
JournalMolecular biology of the cell
Volume18
Issue number8
DOIs
StatePublished - Aug 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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