Polarization of yeast cells in spatial gradients of α mating factor

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Abstract

The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated by factors secreted by the mating partners. Spatial gradients of one of these mating factors, α-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of a-factor, sst2- mutants (which in genetic studies do not discriminate between partners producing different amounts of α-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1% are sufficient for orientation.

Original languageEnglish (US)
Pages (from-to)8332-8336
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
StatePublished - Sep 15 1993

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Mating Factor
Yeasts
Nocodazole
Cell Fusion
Microtubules
Saccharomyces cerevisiae
Intercellular Signaling Peptides and Proteins

Keywords

  • Chemotaxis
  • Orientation

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Polarization of yeast cells in spatial gradients of α mating factor",
abstract = "The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated by factors secreted by the mating partners. Spatial gradients of one of these mating factors, α-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of a-factor, sst2- mutants (which in genetic studies do not discriminate between partners producing different amounts of α-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1{\%} are sufficient for orientation.",
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T1 - Polarization of yeast cells in spatial gradients of α mating factor

AU - Segall, Jeffrey E.

PY - 1993/9/15

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N2 - The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated by factors secreted by the mating partners. Spatial gradients of one of these mating factors, α-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of a-factor, sst2- mutants (which in genetic studies do not discriminate between partners producing different amounts of α-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1% are sufficient for orientation.

AB - The process of cell fusion during mating of the yeast Saccharomyces cerevisiae is mediated by factors secreted by the mating partners. Spatial gradients of one of these mating factors, α-factor, polarized the growth of projections by MATa cells. The site of previous budding did not affect the direction of polarization, and subsequent budding was also polarized if mating factor was removed. Orientation occurred in the presence of nocodazole, suggesting that microtubules were not critical. At extremely low concentrations of a-factor, sst2- mutants (which in genetic studies do not discriminate between partners producing different amounts of α-factor) were able to polarize their projections. The sensitivity of this spatial sensing mechanism in wild-type cells is such that differences in receptor occupancy estimated to be about 1% are sufficient for orientation.

KW - Chemotaxis

KW - Orientation

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M3 - Article

VL - 90

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EP - 8336

JO - Proceedings of the National Academy of Sciences of the United States of America

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