Polarization of Vα24+ Vβ11+ natural killer T cells of healthy volunteers and cancer patients using α-galactosylceramide-loaded and environmentally instructed dendritic cells

Hans J J Van der Vliet, Johan W. Molling, Nobusuke Nishi, Allan J. Masterson, Wendy Kölgen, Steven A. Porcelli, Alfons J M Van den Eertwegh, B. Mary E Von Blomberg, Herbert M. Pinedo, Giuseppe Giaccone, Rik J. Scheper

Research output: Contribution to journalArticle

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Abstract

CD1d-restricted natural killer T (NKT) cells play important regulatory roles in various immune responses. NKT cell-derived T helper (Th) 1 cytokines are important in the induction of antitumor immune responses in mice. Because the CD1d-restricted Vα24+ Vβ11+ NKT cell population in cancer patients is decreased both in size and in its capacity to secrete IFN-γ, therapeutic strategies based on reconstitution of type 1 polarized Vα24+ Vβ11+ NKT cells merit additional investigation. Here, we report the simultaneous strong expansion and type 1 polarization of human invariant Vα24+ Vβ11+ NKT cells using α-galactosylceramide-loaded type 1 dendritic cells and interleukin 15. Type 1 polarized Vα24+ Vβ11+ NKT cells produced high levels of IFN-γ, tumor necrosis factor α, and granulocyte macrophage colony-stimulating factor, and induced strong cytotoxicity in Jurkat cells in an α-galactosylceramide-dependent manner. Importantly, the cytokine profile of Vα24+ Vβ11+ NKT cells that were initially expanded under Th2 polarizing conditions could be reversed to a Thl cytokine profile, indicating the plasticity of the cytokine profile of the human adult Vα24+ Vβ11+ NKT cell population.

Original languageEnglish (US)
Pages (from-to)4101-4106
Number of pages6
JournalCancer Research
Volume63
Issue number14
StatePublished - Jul 15 2003

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Galactosylceramides
Natural Killer T-Cells
Dendritic Cells
Healthy Volunteers
Neoplasms
Cytokines
Interleukin-15
Jurkat Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Population
Tumor Necrosis Factor-alpha

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Polarization of Vα24+ Vβ11+ natural killer T cells of healthy volunteers and cancer patients using α-galactosylceramide-loaded and environmentally instructed dendritic cells. / Van der Vliet, Hans J J; Molling, Johan W.; Nishi, Nobusuke; Masterson, Allan J.; Kölgen, Wendy; Porcelli, Steven A.; Van den Eertwegh, Alfons J M; Von Blomberg, B. Mary E; Pinedo, Herbert M.; Giaccone, Giuseppe; Scheper, Rik J.

In: Cancer Research, Vol. 63, No. 14, 15.07.2003, p. 4101-4106.

Research output: Contribution to journalArticle

Van der Vliet, HJJ, Molling, JW, Nishi, N, Masterson, AJ, Kölgen, W, Porcelli, SA, Van den Eertwegh, AJM, Von Blomberg, BME, Pinedo, HM, Giaccone, G & Scheper, RJ 2003, 'Polarization of Vα24+ Vβ11+ natural killer T cells of healthy volunteers and cancer patients using α-galactosylceramide-loaded and environmentally instructed dendritic cells', Cancer Research, vol. 63, no. 14, pp. 4101-4106.
Van der Vliet, Hans J J ; Molling, Johan W. ; Nishi, Nobusuke ; Masterson, Allan J. ; Kölgen, Wendy ; Porcelli, Steven A. ; Van den Eertwegh, Alfons J M ; Von Blomberg, B. Mary E ; Pinedo, Herbert M. ; Giaccone, Giuseppe ; Scheper, Rik J. / Polarization of Vα24+ Vβ11+ natural killer T cells of healthy volunteers and cancer patients using α-galactosylceramide-loaded and environmentally instructed dendritic cells. In: Cancer Research. 2003 ; Vol. 63, No. 14. pp. 4101-4106.
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