Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

Dennis A. Hall, Douglas C. Maus, Gary J. Gerfen, Souheil J. Inati, Lino R. Becerra, Frederick W. Dahlquist, Robert G. Griffin

Research output: Contribution to journalArticle

344 Citations (Scopus)

Abstract

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

Original languageEnglish (US)
Pages (from-to)930-932
Number of pages3
JournalScience
Volume276
Issue number5314
DOIs
StatePublished - May 9 1997
Externally publishedYes

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Magnetic Resonance Spectroscopy
Electron Spin Resonance Spectroscopy
Muramidase
Microwaves
Glycerol
Free Radicals
Arginine
Electrons
Water
Proteins
nitroxyl

ASJC Scopus subject areas

  • General

Cite this

Hall, D. A., Maus, D. C., Gerfen, G. J., Inati, S. J., Becerra, L. R., Dahlquist, F. W., & Griffin, R. G. (1997). Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Science, 276(5314), 930-932. https://doi.org/10.1126/science.276.5314.930

Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. / Hall, Dennis A.; Maus, Douglas C.; Gerfen, Gary J.; Inati, Souheil J.; Becerra, Lino R.; Dahlquist, Frederick W.; Griffin, Robert G.

In: Science, Vol. 276, No. 5314, 09.05.1997, p. 930-932.

Research output: Contribution to journalArticle

Hall, DA, Maus, DC, Gerfen, GJ, Inati, SJ, Becerra, LR, Dahlquist, FW & Griffin, RG 1997, 'Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution', Science, vol. 276, no. 5314, pp. 930-932. https://doi.org/10.1126/science.276.5314.930
Hall DA, Maus DC, Gerfen GJ, Inati SJ, Becerra LR, Dahlquist FW et al. Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Science. 1997 May 9;276(5314):930-932. https://doi.org/10.1126/science.276.5314.930
Hall, Dennis A. ; Maus, Douglas C. ; Gerfen, Gary J. ; Inati, Souheil J. ; Becerra, Lino R. ; Dahlquist, Frederick W. ; Griffin, Robert G. / Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. In: Science. 1997 ; Vol. 276, No. 5314. pp. 930-932.
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