Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

Dennis A. Hall; Douglas C. Maus; Gary J. Gerfen; Souheil J. Inati; Lino R. Becerra; Frederick W. Dahlquist; Robert G. Griffin

doi:10.1126/science.276.5314.930

Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

Dennis A. Hall, Douglas C. Maus, Gary J. Gerfen, Souheil J. Inati, Lino R. Becerra, Frederick W. Dahlquist, Robert G. Griffin

Research output: Contribution to journal › Article › peer-review

438 Scopus citations

Abstract

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

Original language	English (US)
Pages (from-to)	930-932
Number of pages	3
Journal	Science
Volume	276
Issue number	5314
DOIs	https://doi.org/10.1126/science.276.5314.930
State	Published - May 9 1997
Externally published	Yes

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title = "Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution",

abstract = "Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.",

author = "Hall, {Dennis A.} and Maus, {Douglas C.} and Gerfen, {Gary J.} and Inati, {Souheil J.} and Becerra, {Lino R.} and Dahlquist, {Frederick W.} and Griffin, {Robert G.}",

year = "1997",

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doi = "10.1126/science.276.5314.930",

language = "English (US)",

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T1 - Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

AU - Hall, Dennis A.

AU - Maus, Douglas C.

AU - Gerfen, Gary J.

AU - Inati, Souheil J.

AU - Becerra, Lino R.

AU - Dahlquist, Frederick W.

AU - Griffin, Robert G.

PY - 1997/5/9

Y1 - 1997/5/9

N2 - Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

AB - Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

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JO - Science

JF - Science

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Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

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