Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution

Dennis A. Hall, Douglas C. Maus, Gary J. Gerfen, Souheil J. Inati, Lino R. Becerra, Frederick W. Dahlquist, Robert G. Griffin

Research output: Contribution to journalArticle

347 Scopus citations

Abstract

Large dynamic nuclear polarization signal enhancements (up to a factor of 100) were obtained in the solid-state magic-angle spinning nuclear magnetic resonance (NMR) spectra of arginine and the protein T4 lysozyme in frozen glycerol-water solutions with the use of dynamic nuclear polarization. Polarization was transferred from the unpaired electrons of nitroxide free radicals to nuclear spins through microwave irradiation near the electron paramagnetic resonance frequency. This approach may be a generally applicable signal enhancement scheme for the high-resolution solid-state NMR spectroscopy of biomolecules.

Original languageEnglish (US)
Pages (from-to)930-932
Number of pages3
JournalScience
Volume276
Issue number5314
DOIs
StatePublished - May 9 1997
Externally publishedYes

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ASJC Scopus subject areas

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Cite this

Hall, D. A., Maus, D. C., Gerfen, G. J., Inati, S. J., Becerra, L. R., Dahlquist, F. W., & Griffin, R. G. (1997). Polarization-enhanced NMR spectroscopy of biomolecules in frozen solution. Science, 276(5314), 930-932. https://doi.org/10.1126/science.276.5314.930