Polar tube proteins of microsporidia of the family encephalitozoonidae

Elaine M. Keohane; George A. Orr; Peter M. Takvorian; Ann Cali; Herbert B. Tanowitz; Murray Wittner; Louis M. Weiss

doi:10.1111/j.1550-7408.1999.tb04569.x

Polar tube proteins of microsporidia of the family encephalitozoonidae

Elaine M. Keohane, George A. Orr, Peter M. Takvorian, Ann Cali, Herbert B. Tanowitz, Murray Wittner, Louis M. Weiss

Pathology

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Encephalitozoonidae are microsporidia associated with human infections including hepatitis, encephalitis, conjunctivitis, and disseminated disease. Microsporidia produce a small resistant spore containing a polar tube which serves as a unique vehicle of infection. Polar tube proteins (PTPs) from Encephalitozoon hellem, Encephalitozoon (Septata) intestinalis, and Encephalitozoon cuniculi were purified to homogeneity by HPLC. By SDS-PAGE, the Mr of E. hellem PTP was 55 kDa, while the Mr of E. intestinalis and E. cuniculi PTP was 45 kDa. Polyclonal rabbit antiserum to these purified PTPs localized to polar filaments by immunogold electron microscopy and immunofluorescence, and demonstrated cross-reactivity by both immunoblotting and immunogold electron microscopy. These PTPs have similar solubility properties, hydrophobicity, and proline content to a 43-kDa PTP we have previously purified from Glugea americanus, a fish microsporidium. As the polar tube is critical in the transmission of this organism, further study of PTPs may lead to the development of new therapeutic strategies and diagnostic tests.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	Journal of Eukaryotic Microbiology
Volume	46
Issue number	1
DOIs	https://doi.org/10.1111/j.1550-7408.1999.tb04569.x
State	Published - 1999

Keywords

Microspora
Microsporidia
Polar filament
Polar tube
Polar tube protein

ASJC Scopus subject areas

Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1550-7408.1999.tb04569.x

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abstract = "Encephalitozoonidae are microsporidia associated with human infections including hepatitis, encephalitis, conjunctivitis, and disseminated disease. Microsporidia produce a small resistant spore containing a polar tube which serves as a unique vehicle of infection. Polar tube proteins (PTPs) from Encephalitozoon hellem, Encephalitozoon (Septata) intestinalis, and Encephalitozoon cuniculi were purified to homogeneity by HPLC. By SDS-PAGE, the Mr of E. hellem PTP was 55 kDa, while the Mr of E. intestinalis and E. cuniculi PTP was 45 kDa. Polyclonal rabbit antiserum to these purified PTPs localized to polar filaments by immunogold electron microscopy and immunofluorescence, and demonstrated cross-reactivity by both immunoblotting and immunogold electron microscopy. These PTPs have similar solubility properties, hydrophobicity, and proline content to a 43-kDa PTP we have previously purified from Glugea americanus, a fish microsporidium. As the polar tube is critical in the transmission of this organism, further study of PTPs may lead to the development of new therapeutic strategies and diagnostic tests.",

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AU - Keohane, Elaine M.

AU - Orr, George A.

AU - Takvorian, Peter M.

AU - Cali, Ann

AU - Tanowitz, Herbert B.

AU - Wittner, Murray

AU - Weiss, Louis M.

PY - 1999

Y1 - 1999

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AB - Encephalitozoonidae are microsporidia associated with human infections including hepatitis, encephalitis, conjunctivitis, and disseminated disease. Microsporidia produce a small resistant spore containing a polar tube which serves as a unique vehicle of infection. Polar tube proteins (PTPs) from Encephalitozoon hellem, Encephalitozoon (Septata) intestinalis, and Encephalitozoon cuniculi were purified to homogeneity by HPLC. By SDS-PAGE, the Mr of E. hellem PTP was 55 kDa, while the Mr of E. intestinalis and E. cuniculi PTP was 45 kDa. Polyclonal rabbit antiserum to these purified PTPs localized to polar filaments by immunogold electron microscopy and immunofluorescence, and demonstrated cross-reactivity by both immunoblotting and immunogold electron microscopy. These PTPs have similar solubility properties, hydrophobicity, and proline content to a 43-kDa PTP we have previously purified from Glugea americanus, a fish microsporidium. As the polar tube is critical in the transmission of this organism, further study of PTPs may lead to the development of new therapeutic strategies and diagnostic tests.

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KW - Polar tube protein

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Polar tube proteins of microsporidia of the family encephalitozoonidae

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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