Platelet-endothelial interactions in inflamed mesenteric venules

Paul S. Frenette, Caitlin Moyna, Daqing W. Hartwell, John B. Lowe, Richard O. Hynes, Denisa D. Wagner

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

The selectins are membrane glycoproteins promoting adhesive events between leukocytes, platelets, and endothelial cells. We have previously demonstrated that platelets roll on P-selectin expressed on stimulated endothelium. In this study, we wished to examine the function of both the platelet and endothelial selectins, P- and E-selectins, in mediating platelet-endothelial interactions during inflammation. We demonstrate, using intravital microscopic examination of venules inflamed with tumor necrosis factor-α (TNF-α), that resting platelets interact with both P- and E- selectins and that the leukocyte α(1,3)fucosyltransferases FucT IV and FucT VII do not provide platelets with selectin ligand activity. We also show that after thrombin activation of wild-type (+/+) platelets, platelet P-selectin can mediate interactions on a TNF-α-inducible endothelial ligand. To evaluate the potential role of platelet P-selectin in the recruitment of leukocytes to inflammatory sites, we reconstituted the bone marrow of mice deficient in both P- and E-selectins (P/E-/-) with wild-type (+/+) or P- selectin-deficient (P-/-) bone marrow containing megakaryocytic precursors. Providing +/+ platelets to P/E-/- mice by bone marrow transplantation did not rescue the immunodeficient phenotype, suggesting that platelet P-selectin does not have an active function in the recruitment of leukocytes into inflammatory sites. To participate in inflammatory or hemostatic responses, platelets may use the endothelial selectins.

Original languageEnglish (US)
Pages (from-to)1318-1324
Number of pages7
JournalBlood
Volume91
Issue number4
StatePublished - Feb 15 1998
Externally publishedYes

Fingerprint

Venules
Platelets
P-Selectin
Blood Platelets
E-Selectin
Selectins
Leukocytes
galactoside 3-fucosyltransferase
Bone
Tumor Necrosis Factor-alpha
Bone Marrow
Ligands
Membrane Glycoproteins
Platelet Activation
Hemostatics
Endothelial cells
Bone Marrow Transplantation
Thrombin
Adhesives
Endothelium

ASJC Scopus subject areas

  • Hematology

Cite this

Frenette, P. S., Moyna, C., Hartwell, D. W., Lowe, J. B., Hynes, R. O., & Wagner, D. D. (1998). Platelet-endothelial interactions in inflamed mesenteric venules. Blood, 91(4), 1318-1324.

Platelet-endothelial interactions in inflamed mesenteric venules. / Frenette, Paul S.; Moyna, Caitlin; Hartwell, Daqing W.; Lowe, John B.; Hynes, Richard O.; Wagner, Denisa D.

In: Blood, Vol. 91, No. 4, 15.02.1998, p. 1318-1324.

Research output: Contribution to journalArticle

Frenette, PS, Moyna, C, Hartwell, DW, Lowe, JB, Hynes, RO & Wagner, DD 1998, 'Platelet-endothelial interactions in inflamed mesenteric venules', Blood, vol. 91, no. 4, pp. 1318-1324.
Frenette PS, Moyna C, Hartwell DW, Lowe JB, Hynes RO, Wagner DD. Platelet-endothelial interactions in inflamed mesenteric venules. Blood. 1998 Feb 15;91(4):1318-1324.
Frenette, Paul S. ; Moyna, Caitlin ; Hartwell, Daqing W. ; Lowe, John B. ; Hynes, Richard O. ; Wagner, Denisa D. / Platelet-endothelial interactions in inflamed mesenteric venules. In: Blood. 1998 ; Vol. 91, No. 4. pp. 1318-1324.
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