Plasticity of DNA replication initiation in Epstein-Barr virus episomes

Paolo Norio, Carl L. Schildkraut

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In mammalian cells, the activity of the sites of initiation of DNA replication appears to be influenced epigenetically, but this regulation is not fully understood. Most studies of DNA replication have focused on the activity of individual initiation sites, making it difficult to evaluate the impact of changes in initiation activity on the replication of entire genomic loci. Here, we used single molecule analysis of replicated DNA (SMARD) to study the latent duplication of Epstein-Barr virus (EBV) episomes in human cell lines. We found that initiation sites are present throughout the EBV genome and that their utilization is not conserved in different EBV strains. In addition, SMARD shows that modifications in the utilization of multiple initiation sites occur across large genomic regions (tens of kilobases in size). These observations indicate that individual initiation sites play a limited role in determining the replication dynamics of the EBV genome. Long-range mechanisms and the genomic context appear to play much more important roles, affecting the frequency of utilization and the order of activation of multiple initiation sites. Finally, these results confirm that initiation sites are extremely redundant elements of the EBV genome. We propose that these conclusions also apply to mammalian chromosomes.

Original languageEnglish (US)
JournalPLoS Biology
Volume2
Issue number6
DOIs
StatePublished - 2004

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Human herpesvirus 4
DNA replication
DNA Replication
Human Herpesvirus 4
Viruses
Plasticity
plasmids
Plasmids
DNA
Genes
Genome
genomics
genome
Mammalian Chromosomes
Cells
Molecules
human cell lines
Chromosomes
Chemical activation
chromosomes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Plasticity of DNA replication initiation in Epstein-Barr virus episomes. / Norio, Paolo; Schildkraut, Carl L.

In: PLoS Biology, Vol. 2, No. 6, 2004.

Research output: Contribution to journalArticle

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