Abstract
Changes in the surface morphology of secreting mast cells have been followed by scanning electron microscopy. Mast cells isolated from the rat peritoneal cavity have folds of plasma membrane that form snake-like ridges on their surfaces. Fold length varies considerably from cell to cell, whereas fold width and depth appear to remain relatively constant. To assess the possible relationship between secretory activity and surface folding, a semiquantitative method was used for measuring fold length in control and secreting populations. A positive correlation is found between secretion of histamine and the extent of membrane folds on the mast cell surface. The source of the membrane required for fold formation is probably secretory granule membrane incorporated into the plasma membrane as a result of exocytosis. Furthermore, a distinct cell type devoid of surface folds, designated as a raspberry type cell, is found to occur as an integral part of a normal population of mast cells. This cell type is resistant to stimulation by polymyxin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 690-697 |
| Number of pages | 8 |
| Journal | Journal of Cell Biology |
| Volume | 74 |
| Issue number | 3 |
| State | Published - 1977 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
Cite this
Plasma membrane folds on the mast cell surface and their relationship to secretory activity. / Burwen, S. J.; Satir, Birgit H.
In: Journal of Cell Biology, Vol. 74, No. 3, 1977, p. 690-697.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasma membrane folds on the mast cell surface and their relationship to secretory activity
AU - Burwen, S. J.
AU - Satir, Birgit H.
PY - 1977
Y1 - 1977
N2 - Changes in the surface morphology of secreting mast cells have been followed by scanning electron microscopy. Mast cells isolated from the rat peritoneal cavity have folds of plasma membrane that form snake-like ridges on their surfaces. Fold length varies considerably from cell to cell, whereas fold width and depth appear to remain relatively constant. To assess the possible relationship between secretory activity and surface folding, a semiquantitative method was used for measuring fold length in control and secreting populations. A positive correlation is found between secretion of histamine and the extent of membrane folds on the mast cell surface. The source of the membrane required for fold formation is probably secretory granule membrane incorporated into the plasma membrane as a result of exocytosis. Furthermore, a distinct cell type devoid of surface folds, designated as a raspberry type cell, is found to occur as an integral part of a normal population of mast cells. This cell type is resistant to stimulation by polymyxin.
AB - Changes in the surface morphology of secreting mast cells have been followed by scanning electron microscopy. Mast cells isolated from the rat peritoneal cavity have folds of plasma membrane that form snake-like ridges on their surfaces. Fold length varies considerably from cell to cell, whereas fold width and depth appear to remain relatively constant. To assess the possible relationship between secretory activity and surface folding, a semiquantitative method was used for measuring fold length in control and secreting populations. A positive correlation is found between secretion of histamine and the extent of membrane folds on the mast cell surface. The source of the membrane required for fold formation is probably secretory granule membrane incorporated into the plasma membrane as a result of exocytosis. Furthermore, a distinct cell type devoid of surface folds, designated as a raspberry type cell, is found to occur as an integral part of a normal population of mast cells. This cell type is resistant to stimulation by polymyxin.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017660792&partnerID=8YFLogxK
M3 - Article
C2 - 71300
AN - SCOPUS:0017660792
VL - 74
SP - 690
EP - 697
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 3
ER -