PKa of the essential Glu54 and backbone conformation for subunit c from the H+-coupled F1F0 ATP synthase from an alkaliphilic Bacillus

Iván O. Rivera-Torres, Ray D. Krueger-Koplin, David B. Hicks, Sean M. Cahill, Terry A. Krulwich, Mark E. Girvin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The conformation of the ATP synthase c-subunit and the pKa of its essential E54 residue were characterized in alkaliphilic Bacillus pseudofirmus OF4. The c-subunit folds as a helix-loop-helix, with inter-helical contacts demonstrated by paramagnetic relaxation effects. The E54 pKa of 7.7 is significantly higher than in non-alkaliphiles, which likely prevents proton loss from the c-rotor at high pH. The E54 pKa was unchanged in a mutant, cP51A, that has a severe ATP synthesis defect at high pH only. cP51 must have some structural role that accounts for the mutant defect, such as different subunit-subunit interactions at high pH.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
JournalFEBS Letters
Volume575
Issue number1-3
DOIs
StatePublished - Sep 24 2004

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Bacilli
Bacillus
Conformations
Adenosine Triphosphate
Defects
Protons
Rotors
F1F0-ATP synthase

Keywords

  • Alkaliphile
  • Membrane protein
  • NMR
  • Oxidative phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

PKa of the essential Glu54 and backbone conformation for subunit c from the H+-coupled F1F0 ATP synthase from an alkaliphilic Bacillus. / Rivera-Torres, Iván O.; Krueger-Koplin, Ray D.; Hicks, David B.; Cahill, Sean M.; Krulwich, Terry A.; Girvin, Mark E.

In: FEBS Letters, Vol. 575, No. 1-3, 24.09.2004, p. 131-135.

Research output: Contribution to journalArticle

Rivera-Torres, Iván O. ; Krueger-Koplin, Ray D. ; Hicks, David B. ; Cahill, Sean M. ; Krulwich, Terry A. ; Girvin, Mark E. / PKa of the essential Glu54 and backbone conformation for subunit c from the H+-coupled F1F0 ATP synthase from an alkaliphilic Bacillus. In: FEBS Letters. 2004 ; Vol. 575, No. 1-3. pp. 131-135.
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AU - Hicks, David B.

AU - Cahill, Sean M.

AU - Krulwich, Terry A.

AU - Girvin, Mark E.

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