Pinealocytes in rats

connexin identification and increase in coupling caused by norepinephrine

Juan C. Sáez, Viviana M. Berthoud, Ranjana Kadle, Otto Traub, Bruce J. Nicholson, Michael V. L. Bennett, Rolf Dermietzel

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalBrain Research
Volume568
Issue number1-2
DOIs
StatePublished - Dec 24 1991

Fingerprint

Connexin 43
Connexins
Norepinephrine
Coloring Agents
Messenger RNA
Protein Synthesis Inhibitors
Pineal Gland
Gap Junctions
Incidence
Melatonin
Astrocytes
Connexin 26

Keywords

  • astrocyte
  • Connexin
  • Coupling
  • Gap junction
  • norepinephrine
  • Pinealocyte

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Sáez, J. C., Berthoud, V. M., Kadle, R., Traub, O., Nicholson, B. J., Bennett, M. V. L., & Dermietzel, R. (1991). Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine. Brain Research, 568(1-2), 265-275. https://doi.org/10.1016/0006-8993(91)91407-R

Pinealocytes in rats : connexin identification and increase in coupling caused by norepinephrine. / Sáez, Juan C.; Berthoud, Viviana M.; Kadle, Ranjana; Traub, Otto; Nicholson, Bruce J.; Bennett, Michael V. L.; Dermietzel, Rolf.

In: Brain Research, Vol. 568, No. 1-2, 24.12.1991, p. 265-275.

Research output: Contribution to journalArticle

Sáez, JC, Berthoud, VM, Kadle, R, Traub, O, Nicholson, BJ, Bennett, MVL & Dermietzel, R 1991, 'Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine', Brain Research, vol. 568, no. 1-2, pp. 265-275. https://doi.org/10.1016/0006-8993(91)91407-R
Sáez, Juan C. ; Berthoud, Viviana M. ; Kadle, Ranjana ; Traub, Otto ; Nicholson, Bruce J. ; Bennett, Michael V. L. ; Dermietzel, Rolf. / Pinealocytes in rats : connexin identification and increase in coupling caused by norepinephrine. In: Brain Research. 1991 ; Vol. 568, No. 1-2. pp. 265-275.
@article{c80548750d73439f8ce257a438479f00,
title = "Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine",
abstract = "Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.",
keywords = "astrocyte, Connexin, Coupling, Gap junction, norepinephrine, Pinealocyte",
author = "S{\'a}ez, {Juan C.} and Berthoud, {Viviana M.} and Ranjana Kadle and Otto Traub and Nicholson, {Bruce J.} and Bennett, {Michael V. L.} and Rolf Dermietzel",
year = "1991",
month = "12",
day = "24",
doi = "10.1016/0006-8993(91)91407-R",
language = "English (US)",
volume = "568",
pages = "265--275",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Pinealocytes in rats

T2 - connexin identification and increase in coupling caused by norepinephrine

AU - Sáez, Juan C.

AU - Berthoud, Viviana M.

AU - Kadle, Ranjana

AU - Traub, Otto

AU - Nicholson, Bruce J.

AU - Bennett, Michael V. L.

AU - Dermietzel, Rolf

PY - 1991/12/24

Y1 - 1991/12/24

N2 - Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

AB - Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

KW - astrocyte

KW - Connexin

KW - Coupling

KW - Gap junction

KW - norepinephrine

KW - Pinealocyte

UR - http://www.scopus.com/inward/record.url?scp=0026342973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026342973&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(91)91407-R

DO - 10.1016/0006-8993(91)91407-R

M3 - Article

VL - 568

SP - 265

EP - 275

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -