TY - JOUR
T1 - Pili contribute to biofilm formation in vitro in Mycobacterium tuberculosis
AU - Ramsugit, Saiyur
AU - Guma, Sinenhlanhla
AU - Pillay, Balakrishna
AU - Jain, Paras
AU - Larsen, Michelle H.
AU - Danaviah, Siva
AU - Pillay, Manormoney
N1 - Funding Information:
Acknowledgments We thank Mr Mhlengi Vella Ncube (UKZN) for his contribution to the generation of the ∆mtp mutant; Ms Charissa Naidoo (UKZN) for help with the statistical analysis; and the National Research Foundation (NRF), SA, Medical Research Council (MRC), SA, and College of Health Sciences (CHS), UKZN, for financial support. Mr S. Ramsugit gratefully acknowledges scholarship from the NRF and Canon Collins Trust.
PY - 2013/11
Y1 - 2013/11
N2 - Organized bacterial communities, or biofilms, provide an important reservoir for persistent cells that are inaccessible or tolerant to antibiotics. Curli pili are cell-surface structures produced by certain bacteria and have been implicated in biofilm formation in these species. In order to determine whether these structures, which were suggested to be encoded by the Rv3312A (mtp) gene, have a similar role in Mycobacterium tuberculosis, we generated a Δmtp mutant and a mtp-complemented strain of a clinical isolate of M. tuberculosis and analyzed these strains for their ability to produce pili in comparison to the wild-type strain. Phenotypic analysis by transmission electron microscopy proved the essentiality of mtp for piliation in M. tuberculosis. We then compared biofilm formation of the derived strains in detergent-free Sauton's media. Biofilm mass was quantified spectrophotometrically using crystal violet. Furthermore, we examined mtp gene expression by quantitative real-time PCR in wild-type cells grown under biofilm versus planktonic growth conditions. We found a 68.4 % reduction in biofilm mass in the mutant compared to the wild-type strain (P = 0.002). Complementation of the mutant resulted in a restoration of the wild-type biofilm phenotype (P = 0.022). We, however, found no significant difference between mtp expression in cells of the biofilm to those growing planktonically. Our findings highlight a crucial, but non-specific, role of pili in the biofilm lifestyle of M. tuberculosis and indicate that they may represent an important target for the development of therapeutics to attenuate biofilm formation, thereby potentially reducing persistence.
AB - Organized bacterial communities, or biofilms, provide an important reservoir for persistent cells that are inaccessible or tolerant to antibiotics. Curli pili are cell-surface structures produced by certain bacteria and have been implicated in biofilm formation in these species. In order to determine whether these structures, which were suggested to be encoded by the Rv3312A (mtp) gene, have a similar role in Mycobacterium tuberculosis, we generated a Δmtp mutant and a mtp-complemented strain of a clinical isolate of M. tuberculosis and analyzed these strains for their ability to produce pili in comparison to the wild-type strain. Phenotypic analysis by transmission electron microscopy proved the essentiality of mtp for piliation in M. tuberculosis. We then compared biofilm formation of the derived strains in detergent-free Sauton's media. Biofilm mass was quantified spectrophotometrically using crystal violet. Furthermore, we examined mtp gene expression by quantitative real-time PCR in wild-type cells grown under biofilm versus planktonic growth conditions. We found a 68.4 % reduction in biofilm mass in the mutant compared to the wild-type strain (P = 0.002). Complementation of the mutant resulted in a restoration of the wild-type biofilm phenotype (P = 0.022). We, however, found no significant difference between mtp expression in cells of the biofilm to those growing planktonically. Our findings highlight a crucial, but non-specific, role of pili in the biofilm lifestyle of M. tuberculosis and indicate that they may represent an important target for the development of therapeutics to attenuate biofilm formation, thereby potentially reducing persistence.
KW - Adhesin
KW - Complementation
KW - Curli pili
KW - Gene deletion
KW - Mycobacterium tuberculosis
KW - Persistence
UR - http://www.scopus.com/inward/record.url?scp=84886596483&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886596483&partnerID=8YFLogxK
U2 - 10.1007/s10482-013-9981-6
DO - 10.1007/s10482-013-9981-6
M3 - Article
C2 - 23907521
AN - SCOPUS:84886596483
SN - 0003-6072
VL - 104
SP - 725
EP - 735
JO - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
JF - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
IS - 5
ER -