Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene.

J. Buchert, V. Stefancic, A. G. Doukas, R. R. Alfano, Robert Callender, J. Pande, H. Akita, V. Balogh-Nair, K. Nakanishi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A synthetic retinal having a fixed 11-cis geometry has been used to prepare a nonbleachable analogue of bovine rhodopsin. Marked differences in the picosecond absorption and fluorescence behavior of this analogue at room temperature, compared with that of natural rhodopsin, were observed. This not only indicates that the 11-cis to trans isomerization of the retinal moiety is the crucial primary event in the photolysis of rhodopsin, but also it establishes that this isomerization must occur on the picosecond time scale or faster.

Original languageEnglish (US)
Pages (from-to)279-283
Number of pages5
JournalBiophysical Journal
Volume43
Issue number3
StatePublished - Sep 1983
Externally publishedYes

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Rhodopsin
Fluorescence
Photolysis
Temperature

ASJC Scopus subject areas

  • Biophysics

Cite this

Buchert, J., Stefancic, V., Doukas, A. G., Alfano, R. R., Callender, R., Pande, J., ... Nakanishi, K. (1983). Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene. Biophysical Journal, 43(3), 279-283.

Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene. / Buchert, J.; Stefancic, V.; Doukas, A. G.; Alfano, R. R.; Callender, Robert; Pande, J.; Akita, H.; Balogh-Nair, V.; Nakanishi, K.

In: Biophysical Journal, Vol. 43, No. 3, 09.1983, p. 279-283.

Research output: Contribution to journalArticle

Buchert, J, Stefancic, V, Doukas, AG, Alfano, RR, Callender, R, Pande, J, Akita, H, Balogh-Nair, V & Nakanishi, K 1983, 'Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene.', Biophysical Journal, vol. 43, no. 3, pp. 279-283.
Buchert J, Stefancic V, Doukas AG, Alfano RR, Callender R, Pande J et al. Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene. Biophysical Journal. 1983 Sep;43(3):279-283.
Buchert, J. ; Stefancic, V. ; Doukas, A. G. ; Alfano, R. R. ; Callender, Robert ; Pande, J. ; Akita, H. ; Balogh-Nair, V. ; Nakanishi, K. / Picosecond kinetic absorption and fluorescence studies of bovine rhodopsin with a fixed 11-ene. In: Biophysical Journal. 1983 ; Vol. 43, No. 3. pp. 279-283.
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