Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: An experimental study

Louis F. Amorosa, C. H. Lee, A. B. Aydemir, S. Nizami, A. Hsu, N. R. Patel, T. R. Gardner, A. Navalgund, D. G. Kim, S. H. Park, J. J. Mao, F. Y. Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics. Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically. Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/ hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflects restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle. Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.

Original languageEnglish (US)
Pages (from-to)1637-1643
Number of pages7
JournalInternational Journal of Nanomedicine
Volume8
DOIs
StatePublished - Apr 23 2013
Externally publishedYes

Fingerprint

Bearings (structural)
Autografts
Weight-Bearing
Mesenchymal Stromal Cells
Scaffolds
Allografts
Polymers
Bone
Bone and Bones
Stem cells
Defects
Repair
Bony Callus
Rats
Durapatite
Thigh
Osteogenesis
Stiffness
Hydroxyapatite
Restoration

Keywords

  • Fracture healing
  • Human mesenchymal stem cells
  • Scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

Cite this

Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone : An experimental study. / Amorosa, Louis F.; Lee, C. H.; Aydemir, A. B.; Nizami, S.; Hsu, A.; Patel, N. R.; Gardner, T. R.; Navalgund, A.; Kim, D. G.; Park, S. H.; Mao, J. J.; Lee, F. Y.

In: International Journal of Nanomedicine, Vol. 8, 23.04.2013, p. 1637-1643.

Research output: Contribution to journalArticle

Amorosa, LF, Lee, CH, Aydemir, AB, Nizami, S, Hsu, A, Patel, NR, Gardner, TR, Navalgund, A, Kim, DG, Park, SH, Mao, JJ & Lee, FY 2013, 'Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone: An experimental study', International Journal of Nanomedicine, vol. 8, pp. 1637-1643. https://doi.org/10.2147/IJN.S42855
Amorosa, Louis F. ; Lee, C. H. ; Aydemir, A. B. ; Nizami, S. ; Hsu, A. ; Patel, N. R. ; Gardner, T. R. ; Navalgund, A. ; Kim, D. G. ; Park, S. H. ; Mao, J. J. ; Lee, F. Y. / Physiologic load-bearing characteristics of autografts, allografts, and polymer-based scaffolds in a critical sized segmental defect of long bone : An experimental study. In: International Journal of Nanomedicine. 2013 ; Vol. 8. pp. 1637-1643.
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AU - Lee, C. H.

AU - Aydemir, A. B.

AU - Nizami, S.

AU - Hsu, A.

AU - Patel, N. R.

AU - Gardner, T. R.

AU - Navalgund, A.

AU - Kim, D. G.

AU - Park, S. H.

AU - Mao, J. J.

AU - Lee, F. Y.

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N2 - Background: To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics. Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically. Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/ hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflects restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle. Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.

AB - Background: To address the challenge of treating critical sized intercalary defects, we hypothesized that under physiologic cyclic loading, autografts, allografts, and scaffolds loaded with and without human mesenchymal stem cells (hMSCs) would have different biomechanical characteristics. Methods: Using a rat femoral defect model, 46 rats were assigned to four groups, ie, autograft (n = 12), allograft (n = 10), scaffold (n = 13), and scaffold with hMSCs (n = 11). The scaffold groups used a 5 mm segment of scaffold composed of 80% poly-ε-caprolactone and 20% hydroxyapatite. Rats were sacrificed 4 months postoperatively, and the repairs were assessed radiographically and biomechanically. Results: Autograft and allograft groups exhibited the most bridging callus, while the scaffold/ hMSCs group had more callus than the scaffold repairs. Although signs of radiographic healing did not accurately reflects restoration of mechanical properties, addition of hMSCs on the scaffold enhanced bone formation. The scaffold alone group had significantly lower elastic and viscous stiffness and higher phase angles than other repairs and the contralateral controls. Addition of hMSCs increased the elastic and viscous stiffness of the repair, while decreasing the phase angle. Conclusion: Further comparative analysis is needed to optimize clinical use of scaffolds and hMSCs for critical sized defect repairs. However, our results suggest that addition of hMSCs to scaffolds enhances mechanical simulation of native host bone.

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