Physical and genetic interactions between uls1 and the Slx5-Slx8 SUMO-targeted ubiquitin ligase

Wei Tan, Zheng Wang, Gregory Prelich

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The Slx5-Slx8 complex is a ubiquitin ligase that preferentially ubiquitylates SUMOylated substrates, targeting them for proteolysis. Mutations in SLX5, SLX8, and other SUMO pathway genes were previously identified in our laboratory as genomic suppressors of a point mutation (mot1-301) in the transcriptional regulator MOT1. To further understand the links between the SUMO and ubiquitin pathways, a screen was performed for high-copy suppressors of mot1-301, yielding three genes (MOT3, MIT1, and ULS1). MOT3 and MIT1 have characteristics of prions, and ULS1 is believed to encode another SUMOtargeted ubiquitin ligase (STUbL) that functionally overlaps with Slx5-Slx8. Here we focus on ULS1, obtaining results suggesting that the relationship between ULS1 and SLX5 is more complex than expected. Uls1 interacted with Slx5 physically in to yeast two-hybrid and co-immunoprecipitation assays, a uls1 mutation that blocked the interaction between Uls1 and Slx5 interfered with ULS1 function, and genetic analyses indicated an antagonistic relationship between ULS1 and SLX5. Combined, our results challenge the assumption that Uls1 and Slx5 are simply partially overlapping STUbLs and begin to illuminate a regulatory relationship between these two proteins.

Original languageEnglish (US)
Pages (from-to)771-780
Number of pages10
JournalG3: Genes, Genomes, Genetics
Volume3
Issue number4
DOIs
StatePublished - 2013

Fingerprint

Ligases
Ubiquitin
Mutation
Prions
Immunoprecipitation
Point Mutation
Proteolysis
Genes
Yeasts
Proteins
mamba intestinal toxin 1

Keywords

  • Prion
  • Slx5
  • STUbL
  • Uls1
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Physical and genetic interactions between uls1 and the Slx5-Slx8 SUMO-targeted ubiquitin ligase. / Tan, Wei; Wang, Zheng; Prelich, Gregory.

In: G3: Genes, Genomes, Genetics, Vol. 3, No. 4, 2013, p. 771-780.

Research output: Contribution to journalArticle

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